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What is a gram variable bacteria?
A gram variable bacteria is a type of bacterium that does not consistently stain as either gram-positive or gram-negative. This variability can make it challenging to identify using traditional Gram staining techniques.
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
Difference between the gram stain ans acid fast stain reactions of E. coli?
Gram staining is useful in separating bacteria into two groups: Gram positive or Gram negative. They are separated into these groups based on their cell wall structure. Gram positive bacteria contain a thick layer of peptidoglycan in their cell walls, while Gram negative bacteria contain a very small layer of peptidoglycan (15% or less of what Gram positive cell walls contain). A primary stain is added, such as Crystal Violet, that will stain all of the bacteria. Then, a mordant (such as iodine and potassium iodide) is added to form a complex between the peptidoglycan and the stain, which will make the cell more resistant to decolorization. Then, a decolorizing agent is added, which will remove the primary stain from Gram negative bacteria, but will cause the cell walls in Gram positive bacteria to dehydrate, and therefore, they will retain the primary stain. Finally, a counterstain (typically safranin) is added to distinguish Gram positive from Gram negative. Gram positive cells will be purple, and Gram negative cells will be red if Crystal Violet and Safranin are used.Acid-fast staining is entirely different. Is is used to detect species of bacteria in the genera Mycobacteria and Nocardia. These bacteria are resistant to typical staining procedures, such as Gram staining, due to a thick, waxy lipid layer in the cell wall composed of mycolic acid. Heating of the bacteria with a very strong stain such as carbol-fuchsin is necessary to "melt" this lipid layer, and force the stain through the cell wall. Once the bacteria has cooled, they will be incredibly resistant to decolorization. Non-acid fast bacteria do not contain this mycolic acid layer, and therefore, they will decolorize much easier, and are then stained with a counterstain to distinguish Acid-Fast bacteria from Non-Acid-Fast bacteria.
Is the disease Lyme disease gram stain positive?
Spirochetes are not considered Gram positive or Gram negative. They tend to stain pink due to the last safrinin dye in the staining process, but its cell wall structure should make it Gram negative.
What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
Which thing make gram positive bacteria to get stain?
the smooth part of the cell wall make it to get stain
What is a gram variable bacteria?
A gram variable bacteria is a type of bacterium that does not consistently stain as either gram-positive or gram-negative. This variability can make it challenging to identify using traditional Gram staining techniques.
What is the difference between gram's stain and wright's stain?
Well, sweetheart, Gram's stain is used to differentiate bacteria into two groups based on their cell wall composition - either Gram-positive or Gram-negative. On the other hand, Wright's stain is a Romanowsky stain used in hematology to stain blood cells for microscopic examination. So, in a nutshell, one is for bacteria and the other is for blood cells. Hope that clears things up for you, darling.
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
Difference between the gram stain ans acid fast stain reactions of E. coli?
Gram staining is useful in separating bacteria into two groups: Gram positive or Gram negative. They are separated into these groups based on their cell wall structure. Gram positive bacteria contain a thick layer of peptidoglycan in their cell walls, while Gram negative bacteria contain a very small layer of peptidoglycan (15% or less of what Gram positive cell walls contain). A primary stain is added, such as Crystal Violet, that will stain all of the bacteria. Then, a mordant (such as iodine and potassium iodide) is added to form a complex between the peptidoglycan and the stain, which will make the cell more resistant to decolorization. Then, a decolorizing agent is added, which will remove the primary stain from Gram negative bacteria, but will cause the cell walls in Gram positive bacteria to dehydrate, and therefore, they will retain the primary stain. Finally, a counterstain (typically safranin) is added to distinguish Gram positive from Gram negative. Gram positive cells will be purple, and Gram negative cells will be red if Crystal Violet and Safranin are used.Acid-fast staining is entirely different. Is is used to detect species of bacteria in the genera Mycobacteria and Nocardia. These bacteria are resistant to typical staining procedures, such as Gram staining, due to a thick, waxy lipid layer in the cell wall composed of mycolic acid. Heating of the bacteria with a very strong stain such as carbol-fuchsin is necessary to "melt" this lipid layer, and force the stain through the cell wall. Once the bacteria has cooled, they will be incredibly resistant to decolorization. Non-acid fast bacteria do not contain this mycolic acid layer, and therefore, they will decolorize much easier, and are then stained with a counterstain to distinguish Acid-Fast bacteria from Non-Acid-Fast bacteria.
Is the disease Lyme disease gram stain positive?
Spirochetes are not considered Gram positive or Gram negative. They tend to stain pink due to the last safrinin dye in the staining process, but its cell wall structure should make it Gram negative.
What is the function of safranin in the Gram staining process?
Crystal violet is used first to stain all bacteria purple. However, when you immerse the bacteria then in alcohol, alcohol affects the permeability of the peptidoglycan layer to crystal violet, blocking its exit from gram-positive cells. This way, gram-positive cells remain purple while gram-negative cells are colourless. Safranin red is then used as a counterstain to make the two types of bacteria more differentiable.
What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
What color is coccus under a microscope?
All bacteria, including cocci, are colorless under the microscope unless you use a light filter or a stain. If you use a light filter or a stain then the color depends on which wavelength the filter is for or which type of stain you are using.
What are bacteria that make ammonia?
Bacteria that make ammonia are gram-negative anaerobes.
What is the difference between a simple and a differential stain?
THE DIFFERENTIAL STAIN, SUCH AS THE GRAM STAIN, STAINS THE CELL WALL OF GRAM POSITIVE BACTERIA, DUE TO THE HIGH LEVEL OF PEPTIDOGLYCAN PRESENT IN THE CELL WALL, THESE WILL BE PURPLE IN COLOR. THOSE THAT STAIN GRAM NEGATIVE, DO NOT HAVE AS MUCH PEPTIDOGLYCAN IN THEIR CELL WALL AND WILL RETAIN NEGLIBLE AMOUNTS OF THE CRYSTAL VIOLET AND MORE OF THE SAFFRIN. HENCE THOSE WILL BE GRAM NEGATIVE OR RED TO PINK. THUS THIS TYPE OF STAIN DIFFERENTIATES THE BACTERIA IN TO TWO BASIC CATEGORIES. THIS ASSISTS THE PHYSICIAN IN MAKING A CHOICE REGARDING ANTIBIOTICS TO TREAT THE PATHOGEN WITH. A SIMPLE STAIN JUST SHOWS THE BASIC MORPHOLOGY OF THE BACTERIUM OR BACTERIA PRESENT. YOU MAY WISH TO USE BROMTHYMOL GREEN, CRYSTAL VIOLET... ETC. ALL YOU ARE LOOKING AT IS MORPHOLOGY. HOPE THAT HELPS
Is Mycobacteria gram negative or positive?
If you have done your gram stain properly the mycobacterium will appear to be gram positive, however an acid fast stain is necessary because of the extremely long hydrocarbon chains and the high lipid content in the cell wall. The high lipid content makes the cell wall waxy. Both of these make the cell fairly impermeable to stains.
