answersLogoWhite

0

Ninhydrin solution reacts with amino acids in the developed spots, producing a purple color. This color change makes the spots more visible and helps in their visualization on chromatography materials.

User Avatar

AnswerBot

1y ago

What else can I help you with?

Continue Learning about Natural Sciences

Why you will spray ninhydrin for analysis in chromatography?

Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).


What is locating agent used for?

Locating agent is a substance/liquid that help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.


What are the dark spots called on the earth?

what are the spots on earth main or do the earth have spots what are the spots on earth main or do the earth have spots


Why you are adding marble chips during heating?

Marble chips are added during heating so that the solution or substance can boil evenly. Evenly boiling the solution will cause it not to have heating spots, which can cause an eruption of bubbles. Heating spots are caused when the heat being applied is only getting to the liquid at the bottom of the flask, causing the liquid down there to evaporate more quickly than the upper liquid. The liquid evaporating causes bubbles to shoot through the solution vigorously, disrupting the system. To avoid these heating spots, we add marble chips or boiling chips to induce a nice, even boil.


Are there any great spots on Mercury?

yes two very hot spots

Related Questions

Why you will spray ninhydrin for analysis in chromatography?

Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).


What does it signify when a ladybug lacks spots?

When a ladybug lacks spots, it may signify that it is a different species or that it is a young ladybug that has not developed its spots yet.


What does it signify if a ladybug lacks spots?

If a ladybug lacks spots, it could signify that it is a different species of ladybug or that it is a young ladybug that has not developed its spots yet.


What is the name of the small brownish spots on skin developed by exposure to sunlight?

Tan.


What are the little white spots that can developed near your eye?

They're called milia.


What is locating agent used for?

Locating agent is a substance/liquid that help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.


What is the best face cream for dark spots?

Being a Dermatologist, I would suggest you to go for the best. Dark spots can be due to acne, sun or it can be your age spots. the all in solution is Lifecell. It gives you flawless and glowing skin within a week.


Why do American bulldogs develop black spots on their skin?

I have always heard that American Bulldogs inherited their black spots because of the breeding from Dalmations and certain hunting retrievers such as the English Pointer. Mostly the white dogs are the ones with the spots and as they get older the hair actually grows darker i nthose spots. My AMBD was white as a puppy and as he matued he developed brown freckles where the black spots were on his back. Of course he was covered with black spots but only developed the brown spots on his back. Hope this helps.


What is the best practice for cooling hot spots caused by dynamic loads?

Install a containment solution for dense racks


What is best practice for cooling hot spots caused by dynamic loads?

Install a containment solution for dense racks


What are mud spots?

Mud spots are areas of mud or dirt that have been deposited or splattered onto a surface, such as clothing, shoes, or a vehicle. They can occur from walking or driving through muddy terrain or due to external factors like rain or splashback. Mud spots can be removed by cleaning with water and a cleaning solution.


What are the red spots in sour cream?

Mold.