Cut 2--3 cm long freshly grown roots and transfer them to
freshly prepared fixative, i.e., aceto-alcohol (1:3:: glacial acetic acid : ethanol).
Keep the root tips in the fixative for 24 hours and then transfer them to 70%
ethanol (for preservation and use in future). Onion root-tip cells have a cell
cycle of approximately 24-hour duration, i.e., they divide once in 24 hours,
and this division usually takes place about two hours after sunrise. Therefore,
roots grown on water should be cut only at that time to score maximum
number of dividing cells.
A good substitute stain for looking at onion root tips under a microscope is iodine solution. This stain will help to visualize the cells more clearly by highlighting the cell walls and starch grains present in the root tips.
The cells in an onion tip but not the onion itself are usually actively dividing cells located at the growing tip of the onion root, known as the root cap cells. These cells help protect the delicate meristematic cells responsible for root growth and can often be found in the root zone of plants.
Onion being a monocot plant has fibrous root system
An onion, strictly speaking, is a 'bulb' and is a stem.
You do not need to put an onion root in water, also cutting its roots would not have done any favours to the onion. If you keep the onion in dry and aired environment it will start to sprout naturally and can then be planted.
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Onion root tips
A good substitute stain for looking at onion root tips under a microscope is iodine solution. This stain will help to visualize the cells more clearly by highlighting the cell walls and starch grains present in the root tips.
the root the root No the root is below the ground and the green onion is above the ground which is a young onion. it will eventually grow into a onion.
It is not! An onion is a bulb. A swede, turnip, carrot, and so on, are root.
It is not! An onion is a bulb. A swede, turnip, carrot, and so on, are root.
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Longitudinal sections of onion root tips on commercial slides allow for the observation of different stages of cell division, such as interphase, prophase, metaphase, anaphase, and telophase. This helps in the study of plant cell division processes. Additionally, these slides provide a clear view of the cellular structures and organization within the root tips, aiding in educational purposes for students studying biology.
The onion is a bulb.
Onion being a monocot plant has fibrous root system
Heating onion root tips during mitosis allows for the cells to be fixed and stained, making it easier to observe and study the different stages of cell division under a microscope. The heat helps to stop any ongoing cellular processes that may impact the accuracy of the observations.
HCl is added to onion root tips in the experiment to soften the plant tissue and facilitate the observation of cell division. The acid helps to break down the pectin and other cell wall components, making the cells more permeable and easier to stain. This step allows for clearer visualization of the chromosomes during the mitotic phases when viewed under a microscope.