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Cut 2--3 cm long freshly grown roots and transfer them to

freshly prepared fixative, i.e., aceto-alcohol (1:3:: glacial acetic acid : ethanol).

Keep the root tips in the fixative for 24 hours and then transfer them to 70%

ethanol (for preservation and use in future). Onion root-tip cells have a cell

cycle of approximately 24-hour duration, i.e., they divide once in 24 hours,

and this division usually takes place about two hours after sunrise. Therefore,

roots grown on water should be cut only at that time to score maximum

number of dividing cells.

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