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It is formed by filtration of blood through the fenestrations of the choroidal capillaries in the choroid plexus. It is flows by the active transport of substances (Particularly sodium ions) across the choroid epithelium into the ventricle, water then flows passively across to maintain osmotic balance.

It has also been shown that if the choroid plexus was removed the ventricles still produced substantial quantities of CSF, though less than normal. And is generally considered to be fluid movement from CNS capillaries into the parenchyma of the brain, and from there across the ependymal lining into the ventricle.

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