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The gram stain will stain a bacterium then a particular antibiotic might be effective against it. If the gram stain will not stain that particular bacterium, then that particular antibiotic will not be effective against it.

Thus a gram negative bacterium like TB requires a different antibiotic than a gram positive bacterium.

However, if several have formed a film, it may contain many types and it becomes harder to kill them. That may require a broad based antibiotic.

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