As of now, there are 65 teams in the tournament. Two teams (generally considered to be the worst in the tournament) play an "opening round" game. The winner gets the fourth 16 seed in the first round of the tournament.
The #1 seeds are 108-0 against #16 seeds in the NCAA tournament.
64
64 teams in the NCAA tournament in 2000
The NCAA Men's Basketball Tournament went to 64 teams in 1985.
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64 teams
Sixteen.
In 2000 there were 64 teams that played in the Division I basketball tournament.
64
1985
Since the NCAA implemented the 64 seed tournament format in 1985, the lowest seed to make it to the NCAA championship game was #8 seeded Villanova in 1985.
1985