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Biotic factors, such as competition for food and predation, likely influenced the tortoises Darwin observed by shaping their behavior, feeding habits, and survival rates. Abiotic factors, including climate conditions, vegetation types, and island geography, affected their physical adaptations, such as shell shape and size, which helped them access different food sources. These interactions led to the diversification of tortoise populations across the islands, demonstrating natural selection in response to varying environmental pressures.

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