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How the protein sample without aromatic ring containing aminoacids can be observed under spectrophotometer and at what wave length?
A general protein assay for determining protein concentrations (of proteins with no aromatic amino acids) would be using the Bradford Method. The binding of this Coomassie Brilliant Blue G-250 dye to proteins will cause a shift in the dye's maximum absorbance wavelength. The free dye is red and absorbs at 465nm, but the blue protein-bound dye can be visualized at 595nm.
You will need to construct a standard curve of BSA, for example. Then use the trend line from that curve to determine your protein concentration.
Reference: Bradford, M. 1976, Analytical Biochemistry, vol. 72, p. 248.
What else can I help you with?
Why does protein have an absorbance peak at 280nm?
When a protein in solution is analyzed using UV-visible, a peak at 280 nm is commonly observed. This peak is due to the effect of aromatic rings in the polypeptide chain (from amino acids tryptophan and tyrosine).
What is halotropic isomers?
Halotropic isomers are a type of structural isomerism where the arrangement of atoms in a molecule can undergo rapid rearrangement in solution due to the migration of a hydrogen atom or a π bond. This phenomenon is often observed in aromatic compounds.
What will be observed when some crushed camphor is heated slowly in a china dish What will be observed when some crushed camphor is heated slowly in a china dish What will be observed when some crushe?
When crushed camphor is heated slowly in a china dish, it will undergo sublimation. This means that it will transition directly from a solid to a gas without becoming liquid, producing a strong aromatic vapor. As the heating continues, the camphor will gradually disappear, leaving no residue in the dish. If the cooled dish is observed, the vapor may condense back into solid camphor on the cooler surfaces, forming small white crystals.
Epidermal layer containing visible granules?
The epidermal layer containing visible granules is the stratum granulosum. This layer is responsible for the production of keratin and lipids, which help to strengthen and waterproof the skin. The granules observed in this layer are a result of the keratinocytes undergoing a process called keratinization.
What is the difference of pressure above and below the miniscus of water?
The pressure above the meniscus in water is lower than the pressure below it. This pressure difference results in the upward capillary action observed in narrow tubes containing water.
A property of matter that can be observed and measured with out changing its density?
One property of matter that can be observed and measured without changing its density is its color. The color of a substance can be determined visually or quantitatively using instruments like a spectrophotometer, without altering its density.
Why is cloudiness observed when a urine specimen containing albumin is heated?
cloudiness
What are the characteristic peaks observed in the benzophenone infrared spectrum?
In the benzophenone infrared spectrum, characteristic peaks are typically observed at around 3060-3020 cm-1 for aromatic C-H stretching, 1600-1585 cm-1 for CO stretching, and 750-680 cm-1 for aromatic C-H bending.
What are the characteristic absorption peaks observed in the benzene UV spectrum?
In the benzene UV spectrum, characteristic absorption peaks are typically observed around 180-200 nm due to the presence of aromatic rings in the molecule.
Why aromatic compounds gives yellow colour?
Aromatic compounds contain a delocalized pi electron system that can absorb light in the visible region, leading to the appearance of color. The specific wavelengths of light absorbed by the pi electrons correspond to the yellow part of the spectrum, resulting in the yellow color observed in some aromatic compounds.
What characteristic peaks can be observed in the IR spectrum of a compound containing a CC double bond?
In the IR spectrum of a compound containing a CC double bond, characteristic peaks can be observed around 1650-1600 cm-1 for the CC stretching vibration.
What are the characteristic peaks observed in the benzophenone IR spectrum?
In the benzophenone IR spectrum, characteristic peaks are typically observed around 1700-1600 cm-1 for the carbonyl group (CO) stretch, and around 1600-1500 cm-1 for the aromatic ring stretching vibrations.
Why does protein have an absorbance peak at 280nm?
When a protein in solution is analyzed using UV-visible, a peak at 280 nm is commonly observed. This peak is due to the effect of aromatic rings in the polypeptide chain (from amino acids tryptophan and tyrosine).
What are 4 situations in which evaporation can be observed?
watching it, smelling it, touching it, tasting it, and hearing it. that is how you observe anything, especially evaporation
What is halotropic isomers?
Halotropic isomers are a type of structural isomerism where the arrangement of atoms in a molecule can undergo rapid rearrangement in solution due to the migration of a hydrogen atom or a π bond. This phenomenon is often observed in aromatic compounds.
What is the meaning of ritual?
Of or pertaining to rites or ritual; as, ritual service or sacrifices; the ritual law., A prescribed form of performing divine service in a particular church or communion; as, the Jewish ritual., Hence, the code of ceremonies observed by an organization; as, the ritual of the freemasons., A book containing the rites to be observed.
What will be observed when some crushed camphor is heated slowly in a china dish What will be observed when some crushed camphor is heated slowly in a china dish What will be observed when some crushe?
When crushed camphor is heated slowly in a china dish, it will undergo sublimation. This means that it will transition directly from a solid to a gas without becoming liquid, producing a strong aromatic vapor. As the heating continues, the camphor will gradually disappear, leaving no residue in the dish. If the cooled dish is observed, the vapor may condense back into solid camphor on the cooler surfaces, forming small white crystals.
