How to isolate staphylococcus aureus from freshmeat?

Answer 1

You could run the meat through a blender, strain out the juice, filter that with something along the lines of a 2 um filter then plate 100 ul aliquots on mannitol salt agar plates, incubate at 37C for 24 hours.

If there is growth you have a Staphylococcus, if the media turns yellow you have a S. aureus.

(That's how I'd approach it without looking anything up specifically in a food microbiology reference.)

Answer 2

To isolate Staphylococcus aureus from fresh meat, you can follow these steps:

1. Prepare a sample by homogenizing the fresh meat in a sterile buffer solution.
2. Plate the sample onto a selective agar medium such as Mannitol Salt Agar which inhibits the growth of other bacteria.
3. Incubate the plates at 37°C for 24-48 hours, and look for distinct colonies with characteristic golden-yellow color and positive coagulase test.
4. Confirm the identity of the isolated colonies through biochemical tests or molecular techniques.