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If amylase is added to a sample of potato and you test it with Benedict's reagent 10 minutes later what do you expect of the results to be?
If amylase is added to a sample of potato, which contains starch, it will break down the starch into simpler sugars like maltose. When tested with Benedict's reagent after 10 minutes, you would expect a color change to occur, indicating the presence of reducing sugars. A positive result, typically seen as a color change from blue to green, yellow, orange, or red, suggests that amylase has effectively hydrolyzed the starch into sugars.
What else can I help you with?
What is the common error in using Benedicts?
The common error in using Benedict's reagent is improperly heating the solution. Overheating the reagent can cause it to decompose and give false positive results for reducing sugars, leading to inaccurate conclusions about the presence of sugars in the sample being tested. It is important to follow the specified temperature and time guidelines to ensure accurate measurement of reducing sugars.
Would glucose solution produce a positive Benedicts test?
No, Benedicts reagent will show positive results if the carbohydrate is a reducing sugar. You will know if it is positive if the sample will turn from blue to green then to orange when you are cooling the solution, which is the last step when you are performing the benedicts test for carbohydrates.
Why does the oxidase reagent need to be fresh?
The oxidase reagent needs to be fresh because it contains the enzyme cytochrome c oxidase, which can degrade over time, leading to false-negative results if it is not active. Using fresh reagent ensures the accuracy of the test results.
Why biuret reagent only kept in plastic bottle?
Biuret reagent should only be kept in a plastic bottle because it reacts with glass, which can contaminate the solution and give inaccurate results. Plastic is chemically inert and does not react with the reagent, ensuring the accuracy of the test results.
Why should benzidine reagent be freshly prepared?
Benzidine reagent should be freshly prepared to ensure accuracy and reliability of the results. It is prone to oxidation and degradation over time, which can lead to false positive or negative test outcomes. Freshly prepared benzidine reagent will provide the most accurate and consistent results in tests.
Which is more sensitive test fehlings test or Benedicts test?
Benedict's test is more sensitive than Fehling's test for detecting reducing sugars in a sample. Benedict's reagent has a lower detection threshold and is known to give more accurate results compared to Fehling's reagent.
What are the Benedicts test results for Gatorade?
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What is the common error in using Benedicts?
The common error in using Benedict's reagent is improperly heating the solution. Overheating the reagent can cause it to decompose and give false positive results for reducing sugars, leading to inaccurate conclusions about the presence of sugars in the sample being tested. It is important to follow the specified temperature and time guidelines to ensure accurate measurement of reducing sugars.
Would glucose solution produce a positive Benedicts test?
No, Benedicts reagent will show positive results if the carbohydrate is a reducing sugar. You will know if it is positive if the sample will turn from blue to green then to orange when you are cooling the solution, which is the last step when you are performing the benedicts test for carbohydrates.
Why does the oxidase reagent need to be fresh?
The oxidase reagent needs to be fresh because it contains the enzyme cytochrome c oxidase, which can degrade over time, leading to false-negative results if it is not active. Using fresh reagent ensures the accuracy of the test results.
Why biuret reagent only kept in plastic bottle?
Biuret reagent should only be kept in a plastic bottle because it reacts with glass, which can contaminate the solution and give inaccurate results. Plastic is chemically inert and does not react with the reagent, ensuring the accuracy of the test results.
Why should benzidine reagent be freshly prepared?
Benzidine reagent should be freshly prepared to ensure accuracy and reliability of the results. It is prone to oxidation and degradation over time, which can lead to false positive or negative test outcomes. Freshly prepared benzidine reagent will provide the most accurate and consistent results in tests.
Equation of formaldehyde schiff's reagent benzaldehyde schiff's reagent and acetone schiff's reagent?
Formaldehyde Schiff's reagent: It is a solution of formaldehyde and sulfuric acid, used to detect the presence of aldehydes. The equation involves the formation of a colored compound with aldehydes. Benzaldehyde Schiff's reagent: Benzaldehyde can act as a Schiff's base with primary amines to form imines. The reaction results in the formation of a colored compound. Acetone Schiff's reagent: Acetone can react with a primary amine to form a Schiff's base, leading to the formation of a colored compound.
What is the equation for sucrose and tollens reagent?
The reaction between sucrose and Tollens' reagent results in the formation of a silver mirror. The equation for this reaction is: C12H22O11 (sucrose) + 2Ag(NH3)2OH (Tollens' reagent) → 12Ag (s) + CO2 (g) + H2O (l) + 22NH3 (aq)
An unknown sample is tested with both biuret reagent and benedicts reagent both test result in a blue color what has been learned and why are these called negative results?
That the unknown sample is not a monosaccharide and is does not contain peptide bonds (is not a protein). This is because they both produced negative results because Biuret tests positive in solutions that contain peptide bonds and will turn a violet color. Benedict reagent reacts to monosaccharides and will turn green-reddish orange when a monosaccharide is present. Neither of these things happened so the results are negative.
List two advantages in using the clinitest reagent in preference to Benedict's reagent?
The Clinitest reagent is a self test so it does not need to be performed in a hospital setting or by a nurse or doctor. The Clinitest reagent has proven to be more accurate than the Benedict's reagent.
How do you prepare kedd's reagent?
To prepare Kedde's reagent, mix equal volumes of concentrated hydrochloric acid and 5% potassium dichromate solution. The reagent is used to test for the presence of fatty acids in a substance, where a positive test results in the formation of a layer of orange crystals.
