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What is a repressible operon?
A repressible operon is a type of operon in bacteria where gene expression is usually active but can be turned off when a specific corepressor molecule binds to the repressor protein. This binding causes the repressor to bind to the operator region of the operon, blocking transcription and thus shutting down gene expression. An example of a repressible operon is the trp operon in E. coli, which is involved in tryptophan biosynthesis.
Why lac operon the gene expression is switch off?
explain the regulation of gene expression in lac operon.
True are false the lac operon is a repressible operon?
False. The lac operon is an inducible operon, not a repressible one. It is typically turned off when lactose is absent and activated when lactose is present, allowing the transcription of genes needed for lactose metabolism. In contrast, repressible operons are usually active and can be turned off by the presence of a specific molecule.
Describe how E. coli uses the negative and positive control of the lac operon to economize on RNA and protein synthesis?
if a regulatory protein in its active state turns off the expression of the operon, the operon is said to be negatively regulated by the regulatory protein. if the regulatory protein in its active state truns on the operon, the operon is positively regulated by the regulatory protein. an operon regulated by a repressor is therefore negatively regulated, because the presence of the active repressor prevents transcription of that operon. in contrast, an operon regulated by an activator is positively regulated, because in its active state the activator protein turns on transcription of the operon under its control. the lac operon of E. coli repressor(lac repressor) is synthesized through the activity of the gene lac I, known as regulator gene. the active form of the lac repressor, is a tetramer that contains four copies of the gene I product. in the absence of the inducer, the repressor binds to the lac operator sequence, preventing RNA polymerase from binding to the promoter and transcribing the structural genes. thus the lac operon is negatively regulated associated with the lac operon, there is another site, 16 base pairs upstream of the promoter, which is used for a positive control of the gene expression. this site is called catabolic activator protein site or cyclic AMP(cAMP) protein site or catabolite gene activator(cga) site, because it is utilized for binding of CAP or cga to stimulate gene expression. CAP can bind to this site only when it is bound with cAMP. by binding to it, the CAP-cAMP complex exerts a positive control over the transcription process. it has an effect exactly opposite to that of repressor binding to an operator. yhe effector molecule cAMP determines the effect of CAP on lac operon transcription. presence of glucose inhibits the formation of cAMP and prevents it to bind to CAP
If lactose is present would the lac operon in a bacterial cell be on or off?
If lactose is present, the lac operon in a bacterial cell would be "on." Lactose binds to the repressor protein, causing it to change shape and detach from the operator region of the operon. This allows RNA polymerase to access the promoter and initiate transcription of the genes needed for lactose metabolism. Therefore, the presence of lactose activates the operon.
What is a repressible operon?
A repressible operon is a type of operon in bacteria where gene expression is usually active but can be turned off when a specific corepressor molecule binds to the repressor protein. This binding causes the repressor to bind to the operator region of the operon, blocking transcription and thus shutting down gene expression. An example of a repressible operon is the trp operon in E. coli, which is involved in tryptophan biosynthesis.
What acts as an on and off switch in an operon?
which part of an operon acts as the on or off switch?
What turns the lac operon off and off?
Repressors
Why lac operon the gene expression is switch off?
explain the regulation of gene expression in lac operon.
The lac operon is shut off when what is absent?
The lac operon is shut off when lactose is absent. In the absence of lactose, the repressor protein binds to the operator site, preventing transcription of the lac operon genes.
What turns the lac operon on and off?
The lac operon is turned on when lactose is present in the environment and glucose is scarce. This leads to the activation of the lac repressor protein, allowing the expression of genes involved in lactose metabolism. The lac operon is turned off when lactose is absent or glucose is abundant, which prevents the unnecessary expression of these genes.
True are false the lac operon is a repressible operon?
False. The lac operon is an inducible operon, not a repressible one. It is typically turned off when lactose is absent and activated when lactose is present, allowing the transcription of genes needed for lactose metabolism. In contrast, repressible operons are usually active and can be turned off by the presence of a specific molecule.
A lac repressor turns off the lac genes by binding to?
When the lac repressor binds to the O region, RNA polymerase is prevented from beginning the process of transcription. In effect, the binding of the repressor protein turns the operon "off" by preventing the transcription of its genes. (Prentice Hall Biology Book .....Chapter 12 page 310)
Describe how E. coli uses the negative and positive control of the lac operon to economize on RNA and protein synthesis?
if a regulatory protein in its active state turns off the expression of the operon, the operon is said to be negatively regulated by the regulatory protein. if the regulatory protein in its active state truns on the operon, the operon is positively regulated by the regulatory protein. an operon regulated by a repressor is therefore negatively regulated, because the presence of the active repressor prevents transcription of that operon. in contrast, an operon regulated by an activator is positively regulated, because in its active state the activator protein turns on transcription of the operon under its control. the lac operon of E. coli repressor(lac repressor) is synthesized through the activity of the gene lac I, known as regulator gene. the active form of the lac repressor, is a tetramer that contains four copies of the gene I product. in the absence of the inducer, the repressor binds to the lac operator sequence, preventing RNA polymerase from binding to the promoter and transcribing the structural genes. thus the lac operon is negatively regulated associated with the lac operon, there is another site, 16 base pairs upstream of the promoter, which is used for a positive control of the gene expression. this site is called catabolic activator protein site or cyclic AMP(cAMP) protein site or catabolite gene activator(cga) site, because it is utilized for binding of CAP or cga to stimulate gene expression. CAP can bind to this site only when it is bound with cAMP. by binding to it, the CAP-cAMP complex exerts a positive control over the transcription process. it has an effect exactly opposite to that of repressor binding to an operator. yhe effector molecule cAMP determines the effect of CAP on lac operon transcription. presence of glucose inhibits the formation of cAMP and prevents it to bind to CAP
What is a clue that the lac operon is on?
One clue that the lac operon is on is the presence of lactose in the environment. The lac operon is induced when lactose is available as a substrate for the lac repressor protein, allowing transcription of genes involved in lactose metabolism.
How do off switches work?
Off and on switches connect to a current. An on switch allows the current to run through an object, essentially "turning it on". Off switches cut of this current.
What are features of the lactose operon?
---|CRP|-------------| O |---| Z | Y | A |---CRP: Binding site for activatorO: Operator, binding site for repressorCoding sequences:Z: b-galactosidaseY: lactose permeaseA: thiogalactoside transacetlyase
