0
http://wiki.answers.com/Q/In_dideoxy_sequencing_of_dna_isn%27t_it_so_that_in_each_test_tube_containing_a_separate_ddNTP_apart_from_getting_fragments_ending_at_ddNTPs_you_shall_also_get_the_full_fragments_in_each_case" "http://wiki.answers.com/Q/In_dideoxy_sequencing_of_dna_isn%27t_it_so_that_in_each_test_tube_containing_a_separate_ddNTP_apart_from_getting_fragments_ending_at_ddNTPs_you_shall_also_get_the_full_fragments_in_each_case"
What else can I help you with?
Why might a laboratory be using dideoxy nucleotides?
A laboratory might be using dideoxy nucleotides in DNA sequencing reactions, specifically in the Sanger method. Dideoxy nucleotides lack a 3’ hydroxyl group needed for DNA chain elongation, resulting in termination of DNA synthesis at specific bases. This allows for the determination of the nucleotide sequence of a DNA fragment.
Why do dideoxy nucleotides cause the DNA strand to stop elongating?
Dideoxy nucleotides lack a hydroxyl group at the 3' carbon, which is crucial for the formation of phosphodiester bonds during DNA synthesis. Without this hydroxyl group, the dideoxy nucleotides cannot form a bond with the next nucleotide in the growing DNA strand, leading to termination of strand elongation.
What is the chemical formula for saccharin?
Splenda's chemical formula is 1,6-dichloro-1, 6-dideoxy-BETA-D-fructofuranosyl-4-chloro-4-deoxy-alpha-D-galactopyranoside
Why might a laboratory be using dideoxy nucleotides?
A laboratory might be using dideoxy nucleotides in DNA sequencing reactions, specifically in the Sanger method. Dideoxy nucleotides lack a 3’ hydroxyl group needed for DNA chain elongation, resulting in termination of DNA synthesis at specific bases. This allows for the determination of the nucleotide sequence of a DNA fragment.
Why do dideoxy nucleotides cause the DNA strand to stop elongating?
Dideoxy nucleotides lack a hydroxyl group at the 3' carbon, which is crucial for the formation of phosphodiester bonds during DNA synthesis. Without this hydroxyl group, the dideoxy nucleotides cannot form a bond with the next nucleotide in the growing DNA strand, leading to termination of strand elongation.
What is the chemical formula for saccharin?
Splenda's chemical formula is 1,6-dichloro-1, 6-dideoxy-BETA-D-fructofuranosyl-4-chloro-4-deoxy-alpha-D-galactopyranoside
