When plasmids are used to produce a desired protein, the gene encoding for the protein is inserted into the plasmid. The plasmid is then introduced into a host organism, such as bacteria, which then replicate the plasmid and express the protein. This allows for large-scale production of the desired protein.
Large quantities of protein can be produced by expressing the gene of interest in a bacterial colony such as E. coli. This is typically achieved by cloning the gene into a plasmid, transforming the plasmid into the bacterial cells, and inducing protein expression. The bacterial colony can then be grown in a culture medium optimized for protein production to maximize yields.
Go to http://www.tsienlab.ucsd.edu/Images.htm you will find bacterial colonies will and can express functional fluorescent proteins. As for the plasmid pQE30, it looks to fit into the category of expression at this level.
Organelles inside bacteria do not 'make' GFP. GFP can however be expressed in bacteria by adding the coding sequence for GFP to their genome. This can be done by using a plasmid virus containing the GFP gene sequence. When combined with the plasmid, a very small number of bacteria will take up the plasmid's genome into their own. These bacteria can be isolated by using the 'transform or die' mechanism. Bacteria that do not contain the plasmid are preferentially killed by adding an antibody to the agar. The normal bacteria will not be resistant to this, and will die, but the bacteria which have taken up the plasmid into their genome will also contain an antibody resistance gene. This allows them to live in the presence of the antibody. Since GFP was also added to these bacteria's genome, they will fluoresce.
False. Cells containing a plasmid with an antibiotic resistance gene will survive in the presence of the antibiotic because they can produce the protein that confers resistance, allowing them to withstand the antibiotic's effects.
When plasmids are used to produce a desired protein, the gene encoding for the protein is inserted into the plasmid. The plasmid is then introduced into a host organism, such as bacteria, which then replicate the plasmid and express the protein. This allows for large-scale production of the desired protein.
Large quantities of protein can be produced by expressing the gene of interest in a bacterial colony such as E. coli. This is typically achieved by cloning the gene into a plasmid, transforming the plasmid into the bacterial cells, and inducing protein expression. The bacterial colony can then be grown in a culture medium optimized for protein production to maximize yields.
Incorporation and expression of a plasmid in eukaryotic cells is typically achieved through a process called transfection. This involves introducing the plasmid DNA into the cells using methods such as electroporation or lipid-mediated transfection. Once inside the cell, the plasmid can be expressed by the cell's machinery to produce the desired protein or gene product.
Mix the cDNA with the liver DNA
the plasmid contains a certain gene, which codes for the "Green Flourescent Protein." So you put the plasmid in the bacteria, the plasmid starts making that protein in the bacteria, and boom you've got glowing bacteria. works for bunnies and monkeys too, apparently =)
Genes are used to produce a certain protein or protein components for the cell and as a means to transport this information to the next generation.
Go to http://www.tsienlab.ucsd.edu/Images.htm you will find bacterial colonies will and can express functional fluorescent proteins. As for the plasmid pQE30, it looks to fit into the category of expression at this level.
Organelles inside bacteria do not 'make' GFP. GFP can however be expressed in bacteria by adding the coding sequence for GFP to their genome. This can be done by using a plasmid virus containing the GFP gene sequence. When combined with the plasmid, a very small number of bacteria will take up the plasmid's genome into their own. These bacteria can be isolated by using the 'transform or die' mechanism. Bacteria that do not contain the plasmid are preferentially killed by adding an antibody to the agar. The normal bacteria will not be resistant to this, and will die, but the bacteria which have taken up the plasmid into their genome will also contain an antibody resistance gene. This allows them to live in the presence of the antibody. Since GFP was also added to these bacteria's genome, they will fluoresce.
False. Cells containing a plasmid with an antibiotic resistance gene will survive in the presence of the antibiotic because they can produce the protein that confers resistance, allowing them to withstand the antibiotic's effects.
Plasmids are sections of DNA that are independent of the main chromosome found in prokaryotes. It does not have any proteins other than the ones associated with replication of the plasmid.
Protein components tests measure the amounts and types of protein in the blood.
If you are trying to take a gene from a DNA strand and put insert it into a plasmid, you wouldn't want a restriction enzyme to cut that gene up, or else it would be pretty useless. In other words, you need an enzyme or two that cuts outside that gene so that it can be functional after it's inserted into a plasmid. After your gene of interest is inserted into a plasmid, the plasmid can be put back into a bacterium, then you could genetically engineer plants with it or let the bacterium reproduce and produce many copies of a protein that you had wanted to make in the first place.