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In preparation for electrophoresis procedure enzymes are added to DNA in order to?
In preparation for electrophoresis, enzymes such as restriction enzymes are added to DNA to cut it at specific sequences, resulting in fragments of varying lengths. This fragmentation is essential for analyzing the DNA, as it allows for differentiation based on size during the electrophoresis process. Additionally, enzymes like DNA polymerases may be used to amplify specific regions of interest, enhancing the visibility of the DNA bands after separation.
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Why is glycerol and blue dye added to the gel loading buffer during gel electrophoresis?
Glycerol is added to make the DNA sample denser so that it sinks into the gel and loads properly. Blue dye is added to visualize the sample loading and migration progress during electrophoresis.
Why excess barium chloride is added to the filtrate in the preparation of a dry sample of barium sulphate?
Barium chloride in excess is added to be sure that the reaction is complete.
What is the buffer solution and why is it added to the agarose?
You use a buffer when making agarose gels so that when the gel is used for electrophoresis, the gel is able to conduct electricity. The buffer contains ions from the buffer salts that will facilitate conduction. that was good
Why glycerol used in agarose el electrophoresis?
Glycerol is added to the loading buffer in agarose gel electrophoresis to make the sample denser than the surrounding buffer. This helps the sample sink into the well and prevents it from mixing with the buffer during loading. Additionally, glycerol increases the density of the sample and helps it sink into the gel.
What happens when enzymes are added to starch suspension?
Enzymes, such as amylase, break down starch molecules into simpler sugars like glucose. This process is called hydrolysis. The enzymes speed up the reaction, resulting in the starch suspension becoming thinner or turning into a clear solution as the starch is broken down.
What is the recommended running buffer recipe for a Western blot procedure?
The recommended running buffer recipe for a Western blot procedure typically consists of Tris-glycine buffer with SDS (sodium dodecyl sulfate) added to it. This buffer helps to separate proteins based on their size during electrophoresis.
Why is glycerol and blue dye added to the gel loading buffer during gel electrophoresis?
Glycerol is added to make the DNA sample denser so that it sinks into the gel and loads properly. Blue dye is added to visualize the sample loading and migration progress during electrophoresis.
What is the preparation for a trabeculectomy?
The procedure and its benefits and possible complications are fully explained. Antiglaucoma drugs are prescribed before surgery. Added pressure on the eye caused from coughing or sneezing should be avoided.
Why is a buffer added to the electrophoresis box?
A buffer is added to the electrophoresis box to create a conductive environment for the movement of charged molecules during the process. This helps maintain a stable pH level and ensures consistent results in separating DNA or proteins based on their size and charge.
Why is water added in the preparation of aspirin?
Water is added in the preparation of aspirin to help dissolve the ingredients and facilitate the chemical reactions needed to make the medication.
Why is citric added to juice drink preparation?
Citric acid is added for tartness.
When are enzymes added to your food?
the pancreas excretes enzymes in to the small intestine to break down your food
What is the work of NaCl in soap preparation?
Sodium chloride is added to precipitate the soap.
Why is ethidium bromide added to the agarose gel for electrophoresis?
if the DNA is presnt in the gel, the ethi.bro will bind with it & it will emitt fluoresence.some other fluorescent dyes also available, but they r not showing good results,so ethi.bro is used for DNA
Is fruit sugar the same as added sugar?
No, fruit sugar is naturally occurring in fruits, while added sugar is sugar that is added to food during processing or preparation.
When reporting a staged procedure what modifier is added to the CPT code?
58
Why are enzymes mixed with vinegar less effective?
Enzymes are sensitive to changes in pH and only work with a relatively small pH range. Since vinegar is an acid, the pH of the solution containing the enzymes is changes when vinegar is added.
