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An organism that possesses the citrate lyase enzyme may not test positive on Simmon's citrate agar if it does not effectively utilize citrate under the specific conditions of the test, such as pH or temperature. Additionally, the organism might not be able to grow on the agar due to other metabolic limitations or competition with other microorganisms. This situation is not strictly a false negative, as the test specifically measures the ability to utilize citrate as the sole carbon source, rather than merely the presence of the enzyme. Thus, a negative result could reflect the organism's inability to metabolize citrate in that particular environment.

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3d ago

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Related Questions

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If carbon sources, or nutrients, are carried over into the citrate medium, it will result in a false positive. To avoid this, dilute the inoculum in saline before inoculating the citrate medium.


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