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The electrical potential difference across a cell membrane (the resting potential) is around -65 mV, inside negative.

In nerve cells (neurones) or muscle cells this potential difference is reversed during an action potential. Sodium (Na+) channels open and Na+ ions enter the cell down their concentration gradient. This entry of positive charge depolarises the membrane ie it cancels out the resting pootential and then reverses it, so the potential becomes positive inside and negative outside, giving a potential of about +50mV.

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