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What is blank in spectrophotometer?

A blank is used in order to cancel out or zero the absorbance of all the other components in the sample except the component whose absorbance is to be measured. For example, if you want to measure the absorbance of a solute in water, you will use only water as a blank and the spectrophotometer will subtract the absorbance of water from the spectrum when you measure the absorbance of your solute in water.


Is there any difference between beer Lambert's law and Lambert beer's law?

Beer's law says that absorbance of a molecule or solution is:A = a*b*cwhere A is the absorbance, "a" is the absorptivity (in units of per molar per cm, M-1 cm-1), "b" is the path length (in units of centimeters, cm), and "c" is the concentration (in units of molar, M). The absorptivity, is also commonly known as epsilon.That means that the absorbance is linearly proportional to the thickness of the sample, the concentration of the absorbing medium, and the absorptivity, which is a measure of a given molecule's ability of absorb light.See the Web Links for more information.


Why do you use the wavelength with the maximum absorbance in spectroscopy?

Short answer:Using the maximum wavelength gives us the best results. This is because at the peak absorbance, the absobance strength of light will be at the highest and rate of change in absorbance with wavelength will be the smallest. Measurements made at the peak absorbance will have the smallest error.Long answer: It really depends on what is the largest source of error. Taking the readings at the peak maximum is best at low absorbance, because it gives the best signal-to-noise ratio, which improves the precision of measurement. If the dominant source of noise is photon noise, the precision of absorbance measurement is theoretically best when the absorbance is near 1.0. So if the peak absorbance is below 1.0, then using the peak wavelength is best, but if the peak absorbance is well above 1.0, you might be better off using another wavelength where the absorbance is closer to 1. Another issue is calibration curve non-linearity, which can result in curve-fitting errors. The non-linearity caused by polychromatic light is minimized if you take readings at either a peak maximum or a minimum, because the absorbance change with wavelength is the smallest at those wavelengths. On the other hand, using the maximum increases the calibration curve non-linearity caused by stray light. Very high absorbances cause two problems: the precision of measurement is poor because the transmitted intensity is so low, and the calibration curve linearity is poor due to stray light. The effect of stray light can be reduced by taking the readings at awavelength where the absorbance is lower or by using a non-linear calibration curve fitting technique. Finally, if spectral interferences are a problem, the best measurement wavelength may be the one that minimizes the relative contribution of spectral interferences (which may or may not be the peak maximum). In any case, don't forget: whatever wavelength you use, you have to use the exact same wavelength for all the standards and samples. See http://terpconnect.umd.edu/~toh/models/BeersLaw.htmlTom O'HaverProfessor Emeritus


Is contour interval and vertical interval equal?

Not necessarily but yes, it can be. A contour interval is the difference in elevation between successive contours, while a vertical interval is the distance between any two contours. So yeah, it can be the same sometimes.


What is the difference between barefoot and barefooted?

There is no real difference. Its just a different way of saying the same thing. "Barefooted" is more commonly used in the southern United States than in other places. "Barefoot" is more common in non-southern locales although the slightly more sophisticated "barefoot" is sometimes used in southern locales as well.

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