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Thin layer chromatography is used to separate mixtures and other substances. The accuracy depends upon the person doing the experiment and also what substance is being used. The space from the bottom of the plate needs to be the same on all of them and also use a ruler to make sure. The amount of substance needs to be the same. The same amount of solvent needs to be used as well. Just measure everything that is being used. It is accurate because it can be done quick and cheaper than the others. It is also easy to do and can be extremely accurate when separating drug found in the human stomach in a post mortem.Hope this helps, it helped me get a distinction in a controlled assessment.

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