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There are hydrophobic amino acids and hydrophilic amino acids in protein molecules. After protein folding in aqueous solution, hydrophobic amino acids usually form protected hydrophobic areas while hydrophilic amino acids interact with the molecules of solvation and allow proteins to form hydrogen bonds with the surrounding water molecules. If enough of the protein surface is hydrophilic, the protein can be dissolved in water.
When the salt concentration is increased, some of the water molecules are attracted by the salt ions, which decreases the number of water molecules available to interact with the charged part of the protein. As a result of the increased demand for solvent molecules, the protein-protein interactions are stronger than the solvent-solute interactions; the protein molecules coagulate by forming hydrophobic interactions with each other. This process is known as salting out.
ere are hydrophobic amino acids and hydrophilic amino acids in protein molecules. After protein folding in aqueous solution, hydrophobic amino acids usually form protected hydrophobic areas while hydrophilic amino acids interact with the molecules of solvation and allow proteins to form hydrogen bonds with the surrounding water molecules. If enough of the protein surface is hydrophilic, the protein can be dissolved in water.
When the salt concentration is increased, some of the water molecules are attracted by the salt ions, which decreases the number of water molecules available to interact with the charged part of the protein. As a result of the increased demand for solvent molecules, the protein-protein interactions are stronger than the solvent-solute interactions; the protein molecules coagulate by forming hydrophobic interactions with each other. This process is known as salting out.
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Isolation of genomic DNA from groundnut leaves?
Hello, Its very simple, u can go for the CTAB method , with bit modification, 1) Ensure the youngest possible leaves, very tender enough to crush. 2) Crush the leaf sample in liquid nitrogen. 3) add quickly CTAB buffer to the crushed isolate immediately. 4) the follow Rapid method of isolation.
Is streaking for isolation and streaking for antibiotic sensitivities uses the same technique for streaking?
Yes, both streaking for isolation and streaking for antibiotic sensitivities use the same basic streaking technique. However, the purpose and method of interpretation are different. Streaking for isolation is to obtain pure colonies of a microorganism, while streaking for antibiotic sensitivities is to test the susceptibility of the microorganism to specific antibiotics.
What is Moissan's method for isolation of fluorine?
Fluorine was obtained by the hydrolysis of a mixture of KF2 and HF.
How will prevent salting point in the preparation of aromatic waters?
the methods of preparing aromatic waters are: 1. by distillation 2. by solution 2.1.by direct solution 2.2. by alternate solution
Which is not a method used to keep microorganisms from spoiling food?
Depending on the type of the food many methods of preservation exist: adding sugar, salting, irradiation with gammas, freezing, lyophilisation, pasteurization etc.
Why did they salt meat in World War 1?
Salting food is a method of preserving it.
Why does the unsalted meat is easier to spoiled?
The salt simply acts as a preservative. People have been preserving meat by the salting method for centuries.
What is An alternate method to universal precautions?
BSI, or Body Substance Isolation.
Which is more accurate to measure density Archimedes principle or water displacement method?
They are both the same. displacement method is another name for Archimedes' principle
What are the oldest method of food preservatives?
Drying food in the sun/air. Smoking food in the smoke from a fire. Salting food (and then perhaps smoking it).
Isolation of genomic DNA from groundnut leaves?
Hello, Its very simple, u can go for the CTAB method , with bit modification, 1) Ensure the youngest possible leaves, very tender enough to crush. 2) Crush the leaf sample in liquid nitrogen. 3) add quickly CTAB buffer to the crushed isolate immediately. 4) the follow Rapid method of isolation.
Who is responsible for developing the culture plate method for isolation of bacteria?
Robert Koch
What is the principle involved in the brine floatation method?
the principle of this test are used the method of a qualitative test for the detection of nematode and cestode eggs and coccidia oocysts in the faeces.
Is streaking for isolation and streaking for antibiotic sensitivities uses the same technique for streaking?
Yes, both streaking for isolation and streaking for antibiotic sensitivities use the same basic streaking technique. However, the purpose and method of interpretation are different. Streaking for isolation is to obtain pure colonies of a microorganism, while streaking for antibiotic sensitivities is to test the susceptibility of the microorganism to specific antibiotics.
What are the methods of viral isolation?
One method is to send Viral to his bedroom without supper.
What is Moissan's method for isolation of fluorine?
Fluorine was obtained by the hydrolysis of a mixture of KF2 and HF.
What is the mechanism of salting out?
Salting out is a method where high concentrations of a salt, such as ammonium sulfate, are added to a solution to reduce the solubility of proteins or other molecules. This causes the proteins to aggregate and precipitate out of the solution, allowing for their separation. The salt ions compete with the protein for water molecules, leading to decreased solubility and precipitation.
