Provide two examples of how the Bunsen burner is used during inoculation of a tube culture?

Answer 1

A loop is sterilized by inserting it into a Bunsen burner flame until it is red hot.

1) This will incinerate any contaminating organisms that may be present.

2) This will ensure that viable cells are transferred.

Answer 2

1. The Bunsen burner is used to sterilize the inoculating loop or needle before and after transferring the culture to prevent contamination.
2. The Bunsen burner flame can also be used to heat the neck of the tube culture to create an updraft that prevents airborne contaminants from entering the tube during inoculation.