Strategy for Single cross over and double cross over in gene deletion?

Answer 1

In single crossover gene deletion strategy, a linear DNA fragment with homology to the target gene is introduced, leading to recombination and deletion of the gene. In double crossover strategy, two DNA fragments are introduced flanking the target gene, leading to recombination events resulting in gene deletion. Double crossover strategy is more precise and can avoid potential off-target effects compared to single crossover strategy.