Probably the plasmid is partially uncutted and it is present in his coiled and supercoiled forms. you can add another control as the undigested construct. In addition you can add much more enzyme, change batch of EcoRI and digest overnight with BSA to exclude star activity.
Plasmid isolation has a step called washing step that carried out in the column in which the plasmid DNA are already bind. There are two wash solution, first one endo wash buffer that wash the traces of bacterial membrane remnants such as LPS. Wash buffer two has ethanol wash off any protein contaminants present on the column. These wash steps ensure the purify of isolated plasmid DNA.
A 88,1 gram sample of Ag contain 4,9185.10e23 atoms.
350 g sample of CO contain 12,49 moles.
This depends on the mass of the gold sample.
Matter is formed from atoms. Atoms contain protons, neutrons and electrons. Protons and neutrons contain quarks and gluons.
RNAase is used in plasmid preparation to degrade RNA contaminants present in the sample. This helps to ensure that the isolated plasmid DNA is free from RNA, which can interfere with downstream applications such as PCR or cloning. RNAase treatment is an important step to obtain high-quality plasmid DNA.
For DNA to precipitate down when ethanol added it needs a higher salt concentration which will allow it to precipitate more accurately, hence this salt is given in form of Na acetate which is the best salt for the purpose or else NaCl
Yes, a cat's stool sample can contain traces of litter if the cat ingests it while grooming itself.
Yes, a cat's stool sample can contain litter if the cat ingests some of the litter while using the litter box.
This will give you info
Plasmid isolation has a step called washing step that carried out in the column in which the plasmid DNA are already bind. There are two wash solution, first one endo wash buffer that wash the traces of bacterial membrane remnants such as LPS. Wash buffer two has ethanol wash off any protein contaminants present on the column. These wash steps ensure the purify of isolated plasmid DNA.
350 g sample of CO contain 12,49 moles.
A 88,1 gram sample of Ag contain 4,9185.10e23 atoms.
A pure and fresh prepared uranium sample don't contain plutonium; only the irradiated (in a nuclear reactor) uranium contain plutonium.
which sample contains visible particles
23.3 grams
Mercury I nitrate does not contain sulfur unless the sample is contaminated.