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Given that the two species of swarming gram negative bacilli are Proteus vulgaris and Proteus mirabilis, one must find the tests that differentiate these two organisms. Referencing the Textbook of Diagnostic Microbiology by Mahon, Lehman, and Manuselis, I've found the two major differences are their reactions to citrate and sucrose.

One can use automation or strip testing to discover the organisms reactions to these substances.

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Does proteus vulgaris grow on macconkey agar plate?

It can, but due to the high agar content of MacConkey agar the swarming is slow and restricted. So it is usually quite easy to select non-Proteus colonies for sub-culture if done within 24 hours of incubation.


What is cled agar?

CLED agar, or Cystine-Lactose-Electrolyte-Deficient agar, is a culture medium primarily used for the isolation and differentiation of urinary pathogens, particularly bacteria like Escherichia coli and Enterococcus spp. It is designed to support the growth of most urinary tract infection (UTI) pathogens while inhibiting the growth of some non-pathogenic organisms. The agar contains lactose, which allows for lactose fermentation detection, changing the color of colonies, and aiding in the identification of lactose fermenters versus non-fermenters. Its electrolyte-deficient formulation minimizes swarming of Proteus species, making it easier to identify other bacteria in urine samples.


What is the cellular arrangement of proteus vulgaris?

Proteus vulgaris is a Gram-negative bacterium that typically appears as rod-shaped cells that are often arranged singly or in pairs. They are capable of swarming motility due to their peritrichous flagella arrangement. Additionally, they can exhibit polymorphic shapes, including filamentous forms.


What are two identifying colony characteristics of the genus Proteus?

Proteus species, such as Proteus mirabilis and Proteus vulgaris, typically exhibit two key colony characteristics: they often produce a characteristic swarming motility on solid media, leading to a thick, spreading growth pattern that can resemble a circular or concentric pattern. Additionally, their colonies are usually non-lactose fermenting, appearing as pale or colorless on MacConkey agar, which helps in distinguishing them from lactose-fermenting bacteria.


What shape is proteus mirabilis?

Proteus mirabilis bacteria are rod-shaped (bacillus) and have a single flagellum at one end that assists in movement. They are known for their swarming motility on agar surfaces.