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Increase the density of the gel. This will slow down the denser fragment while speeding up the less dense fragment. The down side is that the denser fragments near the beginning of the gel near the DNA well will be seen as a single band.

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Related Questions

What could be done to resolve two fragments that are nearly the same base-pair length and did not appear on the electrophoresis gel?

You could use other enzymes or use a higher percetage of agarosa to make your gel (so they will have a better chance of separating).


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