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1. They are highly sensitive to changes in physical and chemical conditions surrounding them, therefore, it is hard for mankind to use them.

2. They are easily denatured by even a small increase in temperature and are highly susceptible to poisons and changes in pH. Therefore, the conditions in which they work must be tightly controlled.

3. Enzymes make our works harder when using them as the enzymes substrate mixture must be uncontaminated with other substances that might affect the reaction.

4. The high cost of enzyme isolation and purification discourages their use, especially in areas which currently have an established alternative procedure.

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12y ago

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