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Chromatography is generally used to separate out different orgainc substances and to characterise these substances. The process involves a stationary phase, a mobile phase and either a coloured substance or a UV active stationary phase. In the case of Thin Layer Chromatography (TLC) a drop of the sample to be tested is placed on a plate of silica gel containing a chromaphore (a UV active substance). The end of the plate with the drop of sample is placed into the mobile phase. The mobile phase will travel up the plate taking with it the components of the sample. The smaller the component the further it will travel. This can then be viewed using a UV light.

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What is the difference between partition chromatography and adsorption chromatography?

The partition chromatography involves separation between liquids while adsoption chromatography involves solid and liquid separations. Answer: Partition Chromatography is a liquid liquid extraction which involves two solvents while adsorption chromatography is a liquid solid extraction which involves a solid stationary phase & a liquid mobile phase.


Principle of partition chromatography?

Chromatography in which separation is based mainly on differences be- tween the solubility of the sample components in the stationary phase (gas chromatography), or on differences between the solubilities of the compo- nents in the mobile and stationary phases (liquid chromatography)


What are the different types of chromatography?

there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography


What are some ways chromatography can separate chemicals in a mixture?

Chromatography separates chemicals based on their affinity for a stationary phase and a mobile phase, allowing them to travel at different rates. Different types of chromatography like gas chromatography, liquid chromatography, and thin-layer chromatography utilize different mechanisms such as adsorption, partition, ion exchange, and size exclusion to separate the components in a mixture. By adjusting the conditions like solvent polarity, temperature, and column material, chromatography can effectively separate complex mixtures into individual components.


What difference between dad and uv detector in hplc?

Liquid chromatography (LC) encompasses all chromatographic techniques using liquid mobile phase, including planar chromatography (paper chromatography and thin-layer chromatography) and column chromatography (classical column chromatography, and high-performance liquid chromatography on packed and capillary columns). The term liquid chromatography is nowadays often used as a sinonim for high performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC).

Related Questions

Are Adsorption and partition chromatography types of column chromatography?

Yes,both can performed in columns.


What is the difference between partition chromatography and adsorption chromatography?

The partition chromatography involves separation between liquids while adsoption chromatography involves solid and liquid separations. Answer: Partition Chromatography is a liquid liquid extraction which involves two solvents while adsorption chromatography is a liquid solid extraction which involves a solid stationary phase & a liquid mobile phase.


Examples of partition in technical writing?

what are the examples of partition


Principle of partition chromatography?

Chromatography in which separation is based mainly on differences be- tween the solubility of the sample components in the stationary phase (gas chromatography), or on differences between the solubilities of the compo- nents in the mobile and stationary phases (liquid chromatography)


What are the different types of chromatography?

there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography


What are some ways chromatography can separate chemicals in a mixture?

Chromatography separates chemicals based on their affinity for a stationary phase and a mobile phase, allowing them to travel at different rates. Different types of chromatography like gas chromatography, liquid chromatography, and thin-layer chromatography utilize different mechanisms such as adsorption, partition, ion exchange, and size exclusion to separate the components in a mixture. By adjusting the conditions like solvent polarity, temperature, and column material, chromatography can effectively separate complex mixtures into individual components.


What are examples of chromatography in real life?

Tye Die Water Colors


What are separation technique of solutions?

Examples: distillation, ion-exchange, chromatography, decantation, extraction.


What difference between dad and uv detector in hplc?

Liquid chromatography (LC) encompasses all chromatographic techniques using liquid mobile phase, including planar chromatography (paper chromatography and thin-layer chromatography) and column chromatography (classical column chromatography, and high-performance liquid chromatography on packed and capillary columns). The term liquid chromatography is nowadays often used as a sinonim for high performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC).


What are 3 types of chromatography?

1. thin -layer chromatography 2. gas chromatography 3. liquid chromatography


What are the examples of partition?

Some examples of partition include dividing a hard drive into separate sections to store data, separating a room into smaller areas using temporary walls or dividers, and splitting a file or document into smaller sections for easier organization and management.


What are examples of definition techniques in technical writing?

Examples of definition techniques in technical writing include classification, the process of dividing materials into classes. There are also partition, mechanism, and process.