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For cohesive end ligation, the steps involve digestion of the vector and insert DNA with compatible restriction enzymes, followed by purification of the digested DNA fragments, mix them together in the presence of ligase enzyme, and transform the ligated DNA into a host organism. For blunt end ligation, the steps are similar with the exception that the DNA fragments are generated by restriction enzymes that produce blunt ends, so there is no need to worry about complementary overhangs.

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