Crystal violet, basic fuchsin, and safranin are all dyes which can be used in direct staining because they are cationic which means that they are positively charged. These dyes which are positively charged will react to the bacterial cell wall because the cell wall is negatively charged resulting in a basic stain.
Methylene blue a basic stain is generally used to identify the external morphology of bacteria.The other stain which is used as differential stain and which can also differentiate the baceteia on the basis of their cell wall is gram stain i.e. Crystal voilet and is counter stained with Saffranine
The Leifson staining method is a technique used to stain flagella. The protocol involves fixing the bacteria onto a slide, flooding with tannic acid, applying basic fuchsin, rinsing with distilled water, and then rinsing with copper sulfate. This staining technique highlights the flagella, making them visible under the microscope.
Basic dyes are more effective for bacterial staining because they are positively charged and can easily bind to the negatively charged components of bacterial cells, such as nucleic acids and cell wall components. This strong affinity enhances the visibility of bacteria under a microscope by providing a clear contrast against the background. Additionally, basic dyes typically have higher affinity for cellular structures, allowing for more detailed visualization of bacterial morphology.
This question cannot be answered sensibly. Grams per ml is a measure of density, with dimensions [ML-3]. A kilogram is a measure of mass, with dimensions [M]. Basic dimensional analysis teaches that you cannot convert between measures with different dimensions such as these without additional information.
Yes, you can determine the arrangement of the micoorganism such as chain, random, or spiral as the simple staining allows you to obtained a distinctive contrast between background and its organisms being observed.
No, iodine is not a basic stain. Iodine is commonly used in Gram staining to identify bacteria as either Gram-positive or Gram-negative based on their cell wall composition. It acts as a mordant in the staining process and helps to fix the crystal violet stain in Gram staining.
Crystal violet, basic fuchsin, and safranin are all dyes which can be used in direct staining because they are cationic which means that they are positively charged. These dyes which are positively charged will react to the bacterial cell wall because the cell wall is negatively charged resulting in a basic stain.
The bacterial staining technique where a basic dye is used to stain bacterial cells is called simple staining. In this technique, the positively charged dye binds to the negatively charged bacterial cell structures, making them more visible under a microscope.
The basic unit for mass is grams. The symbol for grams is g.
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A stain selective for cell nuclei, usually based on the binding of a basic dye to DNA or to nucleohistone.
The description matches eosinophils, a type of white blood cell. Eosinophils are characterized by dark-staining granules that can be visualized under a microscope by basic dyes such as eosin. They play a role in allergies and parasitic infections.
H&E staining is the classic staining used for basic cellular cytology. The "H" turns acidic structures (such as DNA) blue and the "E" turns the proteins red.
Grams
addition, subtraction, multiplication, and division.