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To confirm Crassulacean Acid Metabolism (CAM) in plants, biochemical analyses typically include measuring the accumulation of organic acids, particularly malate, during the night and their decarboxylation during the day. Additionally, monitoring changes in photosynthetic gas exchange, such as CO2 uptake and water loss, can be crucial. The use of isotopic labeling to trace carbon fixation pathways and analyzing enzyme activities related to CAM, like phosphoenolpyruvate carboxylase (PEPC) and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), also supports the identification of CAM in plants.

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2w ago

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