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Generally, the biotic factors present in a grassland are "Competition" and "Predation", as well as "Mutualism" which can be linked with the first factor. Competition for resources such as food, water, light exposure etc will exist between organisms within a species (intraspecific) and outside a species too (interspecific). Mutualism, which can be linked with competition, involves having an organism (usually a parasite or bacterium) living on a host to survive, but providing the host with a substance it needs. For instance, Ruminent cows are unable to break down (digest) cellulose which is present in the grass they feed on (because they lack the enzymes), but instead the cellulolytic bacteria, which reside in the cow's "second stomach", which is actually an extension of the oesophagus, do the job for the cow. This is an example of mutualism, where the cow is provided with digested cellulose, and the bacteria are provided with a habitat.

Predation involves the natural hunting, killing and feeding of predators on prey, which is what every organism must experience in their lifetime and is present everywhere in large and small degrees.

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