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DNA ladder is made up of a phosphate group, 5-carbon sugar, and nitrogen bases. 5-carbon sugar is deoxiribose in DNA. these nitrogen bases are adenine, guanine, thymine, and cytosine. in these nitrogen bases, adenine bonds with thymine, and guanine bonds with cytosin. In this DNA ladder, the phosphate group and 5-carbon sugar act as two sides of the ladder and the middle of the ladder is nitrogen pair bases.-SALMA ABRAHIM(:

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