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Barfoed's reagent is primarily used to differentiate between monosaccharides and disaccharides in carbohydrate analysis. It contains copper acetate in acetic acid, and when heated with reducing sugars, it forms a red precipitate of cuprous oxide, indicating the presence of monosaccharides. Disaccharides typically do not react under these conditions due to their more complex structure. This reagent is particularly useful in biochemical assays and food analysis to identify sugar types.

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1mo ago

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