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When the ATP synthase molecule opens, it allows protons (H+) to flow through its channel, driven by the proton gradient established across the membrane. This flow of protons causes the rotor portion of ATP synthase to spin, which mechanically drives the conversion of adenosine diphosphate (ADP) and inorganic phosphate (Pi) into adenosine triphosphate (ATP). This process is a crucial part of cellular respiration and photosynthesis, enabling cells to harness energy for various biological functions.

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