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Gram positive are purple. Just remember Positive+Purple. Gram negative are a reddish color from the safranin. After using the crystal violet, the grams iodine acts as a mordant to hold color on cell wall. When using the alcohol, the gram positive will stay purple while the gram negative will become clear. This is due to the outer cell wall of the gram negative specimen (lipopolysaccharide) basically being stripped by the alcohol, so now you have a clear specimen again. When you add the safranin it sticks to the gram negative cell wall, hince the red/pink color.
What else can I help you with?
Could a negatively charged dye stain a bacteria?
The effect on the bacteria depends if the stain is an acidic or basic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the bacterial cell, which is slightly negatively charged.
What is the stain most useful in clinicians to decide for bacterial infection?
Methylene blue a basic stain is generally used to identify the external morphology of bacteria.The other stain which is used as differential stain and which can also differentiate the baceteia on the basis of their cell wall is gram stain i.e. Crystal voilet and is counter stained with Saffranine
Why does some bacteria stain purple and some red?
The purple stained bacteria are called gram positive bacteria and retain the stain after washing by having a thick cell wall one one layer of a heavily crossed linked material called peptidoglycan. ( Google that ) The red stained bacteria are gram negative because they posses two thinner layers of peptidogylcan cell wall that that do not hold the gram stain and it is mostly washed away in rinsing the bacterial samples.
Can Methylene blue used to stain bacteria?
Yes because Methylene Blue is a symple stain which allows the staining of Cocci. The only thing that is done with the stain is to show the morphology of the bacteria, so one could tell the shape, size, and, arrangement.
What does gram negative on a serratia marcescens gram stain mean?
Bacteria are gram positive or gram negative. Serratia happens to be a gram negative bacteria. They appear pink on a gram stain. Gram positive bacteria stain to a purple color on a gram stain. We can classify and ID bacteria using their gram stain and shape. Some antibiotics only work on gram negative bacteria and some only work on gram positive bacteria. It helps a doctor know which antibiotic to use.
How do you know that it is a negative or a positive bacteria?
By which type of stain colors it.
What is the advantage of the gram stain over the simple stain?
gram staining differentiates between gram negative and gram positive bacteria by showing different colors. it shows blue or purple like color for gram positive bacteria and red color for gram negative bacteria. where as simple stain gives the same color to all the types of bacteria. hence it is difficult to differentiate between them.
Do acid-fast bacteria stain gram-negative when subjected to the gram stain?
No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.
What is the gram stain of H5N1?
The Gram stain is used for bacteria and not for viruses.
Which stain sticks to the peptidoglycan in the cell wall of bacteria?
The stain that sticks to the peptidoglycan in the cell wall of bacteria is called crystal violet.
Is maneval's stain an acid stain?
Yes, Maneval's stain is an acid-fast stain used in microbiology to detect acid-fast bacteria such as Mycobacterium species. It involves using acid-alcohol to decolorize non-acid-fast bacteria while acid-fast bacteria retain the stain due to their waxy cell wall.
Why isn't the Gram stain used on acid-fast bacteria?
The Gram stain is not effective on acid-fast bacteria because their cell walls have a waxy layer that prevents the stain from penetrating.
Could a negatively charged dye stain a bacteria?
The effect on the bacteria depends if the stain is an acidic or basic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the bacterial cell, which is slightly negatively charged.
Does yogurt stain gram positive or gram negative?
Yogurt contains mostly lactic acid bacteria, which are Gram-positive bacteria. This means they will stain purple under a Gram stain.
What is the stain most useful in clinicians to decide for bacterial infection?
Methylene blue a basic stain is generally used to identify the external morphology of bacteria.The other stain which is used as differential stain and which can also differentiate the baceteia on the basis of their cell wall is gram stain i.e. Crystal voilet and is counter stained with Saffranine
What are the available table stain colors for the furniture collection?
The available table stain colors for the furniture collection are oak, mahogany, cherry, and espresso.
Does Oxiclean effectively kill bacteria?
Oxiclean is not specifically designed to kill bacteria. It is a stain remover and laundry booster that helps to lift stains and brighten colors in clothing. If you are looking for a product to kill bacteria, you may want to consider using a disinfectant or antibacterial cleaner instead.
