DNA polymerase is responsible for the process of DNA replication, during which a double-stranded DNA molecule is copied into two identical DNA molecules. Scientists have taken advantage of the power of DNA polymerase molecules to copy DNA molecules in test tubes via a polymerase chain reaction, also known as PCR.
Creative Biogene
The backbone of the DNA strand (referred to as phosphate-deoxyribose backbone) is made of alternating phosphate and sugar residues (2'-deoxyribose). The sugars are joined together by phosphate groups via phosphodiester bonds between adjacent sugar rings. The double helix is stabilized by hydrogen bonds between the bases attached to the two strands. A base attached to a sugar residue, which in turn attached to a phosphate group is referred to as a nucleotide.
DNA Helicase unwinds and unzips the DNA. It separates the two strands of DNA so DNA replication can occur.
Recombinant DNA is a new form of DNA because it is created via introduction of the relevant DNA into the existing organismal DNA.
The biologist is studying the process of DNA replication, which is the process by which DNA is copied to produce two identical DNA molecules. This process involves DNA polymerase, which synthesizes a new DNA strand by adding complementary nucleotides to the original DNA template strand.
Ligase is used to join DNA strands together
The backbone of the DNA strand (referred to as phosphate-deoxyribose backbone) is made of alternating phosphate and sugar residues (2'-deoxyribose). The sugars are joined together by phosphate groups via phosphodiester bonds between adjacent sugar rings. The double helix is stabilized by hydrogen bonds between the bases attached to the two strands. A base attached to a sugar residue, which in turn attached to a phosphate group is referred to as a nucleotide.
by DNA fingerprinting method , DNA-DNA hybirdization or DNA sequencing. to know the sequence of DNA
DNA is DNA it can not be changed.
The four enzymes involved in DNA replication and repair are DNA polymerase, DNA helicase, DNA ligase, and DNA primase. DNA polymerase synthesizes new DNA strands, DNA helicase unwinds the double helix, DNA ligase joins the Okazaki fragments on the lagging strand, and DNA primase synthesizes RNA primers for DNA polymerase to begin replication.
DNA replication is semi-conservative i.e during replication one strand comes fron the parent and another is made complementary to it .The enzymes which helps in its duplication r collectively called REPLIOSOMES or multiple replication enzyme complex. for replication first unwinding should take place and this is done by breaking hydrogen bonding between the nitrogen bases with the help of HELICASES then SSPBs( single strand binding proteins) keep the strands in structured manner so that easy polymerisation of nucleotides should take place and finally TOPOISOMERASES introduce negative coils in the strand by introducing a nick in one of the strands and rejoins the cut again to remove tension on the strand .But It is actually with the help of DNA polymerases and RNA primer that DNA is able is able to replicate.DNA polymerases r of three types namely DNA POLYMARASE 1: IT IS INVOLVED IN REMOVAL OF RNA PRIMER IN THE TEMPLATE STRAND OR PARENT STRAND. it should b noted that no DNA polymerase can initiate DNA replication .it is the RNA primer which initiates DNA replication .it start on adding rna which is then removed by DNA polymerase 1 .to initiate DNA replication is not easy for DNA polymerase as they can commit a lot of mistakes n hence rna primer form rna portion only to make a perfect DNA DNA POLYMERASE2: IT IS TO REPAIR THE DNA DURING INJURY DNA POLYMERASE3:WHICH IS THE REAL REPLICATING ENZYME All of them have endonuclease activity in 3'-5' directionwhich help in proof reading or editing function when its frequency of making mistake is 1 out of 10,000 nucleotides.DNA formation has direction always from 5'-3' since the two strands r anti-parralel hence one(LEADING STRAND) is formed in continuous manner while other(LAGGING) in fragments called OKAZAKI FRAGMENTS
DNA Helicase unwinds and unzips the DNA. It separates the two strands of DNA so DNA replication can occur.
IT can be called * DNA duplication * DNA reproduction * DNA imitation
DNA
DNA synthesis or DNA replication
It is how the DNA is constructed.
genes are in DNA
Recombinant DNA is a new form of DNA because it is created via introduction of the relevant DNA into the existing organismal DNA.