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If we use the word "chromatid", there are always two. (As with twins!)

In a eukaryotic cell immediately after cell division (mitosis or meiosis), each chromosome contains only one DNA molecule.

If the cell is preparing for a subsequent division, then midway through interphase the DNA replicates. Now there are two molecules of DNA in each chromosome.

At the start of the following division, in prophase, the chromosomes condense, and the two DNA molecules, with their many associated proteins, become visible under a light microscope as sister-chromatids.

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