Methylene blue is used to stain animal cells, such as human cheek cells, to make their nuclei more observable.
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Methylene blue is a membrane-permeable dye that can enter the cell and bind to cellular components, such as proteins and nucleic acids. This binding can alter the osmotic properties of the cell and affect its solute potential. Methylene blue can also disrupt the electron transport chain in mitochondria, leading to changes in cellular metabolism and solute potential.
If methylene blue is blue, it means that the compound is in its oxidized state (methylene blue) and has accepted electrons. Methylene blue can exist in both oxidized (blue) and reduced (colorless) forms depending on its redox state.
Methylene blue can inhibit the growth and reproduction of paramecium by disrupting cellular respiration and other metabolic processes. It interferes with the electron transport chain in mitochondria, affecting energy production in the cell and ultimately leading to its death.
Methylene blue is a solid, odorless, dark green powder at room temperature. In water this chemical compound turns into a blue solution. When methylene blue is reacted with yeast cells it inhibits the respiration which stops the cells from using hydrogen ions to release energy.
When you added methylene blue to the living yeast cell, the methylene blue is actually a dye so you could see the cells, therefore, the yeast cell became blue. It is much the same with hair dye. If you have a bottle of blue hair dye, it will dye your hair blue.
because methylene blue turns colourless when it is reduced by hydrogen. during respiration hydrogen is produced and instead of reducing NAD, it reduces methylene blue and turns methylene blue colourless. if methylene blue goes from blue to colourless then this shows that the cell is respiring as it is producing a suffiecient amount of hydrogen to decolourise methylene blue
Methylene blue discolouration measures the rate at which a material (usually soil) can reduce methylene blue dye. It is used to assess the dehydrogenase activity of microorganisms present in the material, providing an indication of soil microbial activity and overall soil health.
Methylene blue can act on only dead yeast cells because it is able to penetrate the cell membrane easier when the cell is dead, allowing it to enter and stain the cell. In live yeast cells, the cell membrane is intact and acts as a barrier, preventing methylene blue from entering and staining the cell.
Methylene blue is used to stain animal cells, such as human cheek cells, to make their nuclei more observable. Also used to staining the blood film and used in cytology. It gives a blue coloration to the human cheek cell.
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When a tomato cell is stained with methylene blue, organelles such as the nucleus, vacuoles, and possibly some smaller structures like mitochondria or chloroplasts may become visible under the microscope. Methylene blue tends to bind to nucleic acids and can highlight these structures within the cell.
Methylene blue is necessary for various applications, such as in medical diagnostics, staining biological tissues, and as a medication to treat methemoglobinemia. It is also used as a redox indicator in chemical reactions and as a dye in various industries. In microscopy, methylene blue can help highlight cell structures for better visualization.
When staining a cheek cell with methylene blue, you can observe the cell's nucleus as a distinct blue color. The stain highlights the nucleus due to its affinity to bind with DNA. The rest of the cell may appear pale blue or colorless in comparison.
NADH
Methylene blue is a membrane-permeable dye that can enter the cell and bind to cellular components, such as proteins and nucleic acids. This binding can alter the osmotic properties of the cell and affect its solute potential. Methylene blue can also disrupt the electron transport chain in mitochondria, leading to changes in cellular metabolism and solute potential.