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Agar, in agar plates used in culturing bacteria and other microorganisms, consists of a gelling agent, and is an unbranched polysaccharide obtained from the cell walls of some species of red algae, primarily from the genera Gelidium and Gracilaria, or seaweed. As very few bacteria are able to decompose the actual agar itself, nutrients such as glucose must be added to the agar mixture to allow bacterial growth. It is these various nutrients that the bacteria feed on to survive.

The typical agar plate contains:

# A carbon source such as glucose for bacterial growth # Water # Agar # Various salts needed for bacterial growth # A source of amino acids and nitrogen (e.g., beef, yeast extract) This particular mix is an undefined medium because the amino acid source contains a variety of compounds with the exact composition being unknown. In this case, most bacteria will be using all the above except 3. Agar for nutrition.

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