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What is the basic chemistry involved in the FeCl3 test for phenylketonuria?

The FeCl3 test for phenylketonuria is based on the reaction between phenylpyruvic acid in urine and FeCl3, forming a green-colored complex due to the chelation of the iron ion. This reaction is specific for detecting phenylketonuria because phenylpyruvic acid is elevated in individuals with this disorder, leading to the formation of the colored complex. The color intensity of the complex formed is directly proportional to the concentration of phenylpyruvic acid present in the sample.


What is the chemical equation between phenol and ferric chloride?

We use Ferric Chloride (FeCl3) in the lab to test for the presence (or in some case absence) of phenols, although some enols will also yield positive results. The OH (hydroxy group) which is attached directly to an aromatic nucleus (Benzene, for example) is detected by the Ferric chloride. It is important to note that alcohols do not undergo the reaction. Phenols will typically yield dramatic purple,blue,red or green color as an indication of a positive test. FeCl3 will also indicate the presence of aliphatic acids (that is, non-aromatic organic acids, such as Acetic acid) turning the solution a yellow color. Aromatic acids will test as a beige-tan color. Enols will also show a characteristic yellow color. It is also important to understand that not all phenols will test positively in the presence of the FeCl3 reagent. A good example of this is in highly hindered phenols which do not yield positive tests. However, a negative test is not completely conclusive, so other tests can be preformed to verify the absence of a phenol (such as NMR and IR spectroscopy) We use Ferric Chloride (FeCl3) in the lab to test for the presence (or in some case absence) of phenols, although some enols will also yield positive results. The OH (hydroxy group) which is attached directly to an aromatic nucleus (Benzene, for example) is detected by the Ferric chloride. It is important to note that alcohols do not undergo the reaction. Phenols will typically yield dramatic purple,blue,red or green color as an indication of a positive test. FeCl3 will also indicate the presence of aliphatic acids (that is, non-aromatic organic acids, such as Acetic acid) turning the solution a yellow color. Aromatic acids will test as a beige-tan color. Enols will also show a characteristic yellow color. It is also important to understand that not all phenols will test positively in the presence of the FeCl3 reagent. A good example of this is in highly hindered phenols which do not yield positive tests. However, a negative test is not completely conclusive, so other tests can be preformed to verify the absence of a phenol (such as NMR and IR spectroscopy)


Conclusion for non reducing sugars test?

The non-reducing sugars test is negative if there is no color change after performing the test. This indicates the absence of non-reducing sugars such as sucrose in the sample.


What 2 solutions can you use to test for alkenes?

You can use a litmus test it will tell you if it is alkane and will tell you the PH of the alkane metal.


What does 'the test results were not conclusive' mean?

It means that the results weren't strong enough to make a conclusion. In other words, the issue that the test was investigating has not been fully resolved. When used in the medical sense it means that the test has not highlighted an exact diagnosis or that it hasn't confirmed or denied what was being tested for.

Related Questions

What test would you use to confirm that you have a solution of FeCl3?

You can use a precipitation test by adding NaOH solution to identify FeCl3, which forms a red-brown precipitate of iron hydroxide. Another test involves adding potassium ferrocyanide, which forms a deep blue precipitate of ferric ferrocyanide. Both of these confirm the presence of FeCl3.


Salicyclic acid gives a positive test with FeCl3?

This is a commonly used test to detect the presence of phenolic hydroxyl groups in organic compounds. The reaction between salicylic acid and FeCl3 forms a complex with a purple color, indicating the presence of phenolic groups in the compound.


What would be seen if you analyze salicylic acid in FeCl3 test?

If salicylic acid is tested with FeCl3, a violet color will be observed indicative of the presence of phenols in the compound. This test is commonly used to identify the presence of phenolic functional groups in organic compounds.


How much gram of fecl3 to be used in preparing fecl3 solution for testing citric acid in semen?

The amount of FeCl3 needed depends on the concentration of the FeCl3 solution required for the test. Typically, a 2-5% solution of FeCl3 is used. To make a 100mL of 2-5% FeCl3 solution, you would need to dissolve 2-5 grams of FeCl3 in distilled water. The exact amount can be calculated using the formula: (desired % concentration/100) x volume of solution needed x molar mass of FeCl3.


What is the conclusion of silt test?

conclusion silt test


What is the basic chemistry involved in the FeCl3 test for phenylketonuria?

The FeCl3 test for phenylketonuria is based on the reaction between phenylpyruvic acid in urine and FeCl3, forming a green-colored complex due to the chelation of the iron ion. This reaction is specific for detecting phenylketonuria because phenylpyruvic acid is elevated in individuals with this disorder, leading to the formation of the colored complex. The color intensity of the complex formed is directly proportional to the concentration of phenylpyruvic acid present in the sample.


How neutral fecl3 solution prepare?

Take 1 ml FeCl3 solution. add ammonia solution drop wise till brown precipitate just form. Now again add FeCl3 solution till brown precipitate just dissolve. This is your Neutral FeCl3 solution.


Give a conclusion if rigidity modulus optained by tortion test is low?

If rigidity obtained by a torsion test is low, that means that the item is likely to fracture. A torsion test will tell a person how easily something will break.


What is conclusion if urine c s test shows no growth?

What is conclusion if urine c s test shows no growth


What is the difference between results and conclusion?

Results is the information that came out of an expirement or a test. A conclusion would be what you infered from the results, like a result of a test is an A+, your conclusion would be, that person is smart.


If your partner test postitive for HIV and you test negative what conclusions can be made about that?

Conclusion: Your partner has hiv and you don't.......yet.


What does form hypothesis mean?

A hypothesis is a conclusion. To form a hypothesis one would test theories in order to come up with an accurate conclusion.