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The buildup of oxygen and the emergence of the first eukaryotes primarily occurred during the Proterozoic Eon, particularly in the Great Oxidation Event around 2.4 billion years ago. This period saw a significant increase in atmospheric oxygen due to photosynthetic cyanobacteria. Eukaryotes, which are more complex cells with membrane-bound organelles, are believed to have evolved during this eon, likely in the latter part, around 1.6 billion years ago.

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