...are:
-the attraction to the paper
-the solubility of the pigments
-the size of the particles
posted by Huguito Doutre
o0o
The temperature of the room could be an uncontrolled variable in paper chromatography of pigments, as it can affect the rate at which the solvents evaporate and the separation of the pigments on the paper. Temperature fluctuations could lead to inconsistent results in the chromatography process.
Distillation
Hundreds of different pigments can be identified through the separation process using techniques like chromatography or spectrophotometry. Pigments such as chlorophyll a, chlorophyll b, carotenoids, and anthocyanins are commonly detected in plants.
Yes, the presence of different pigments can be detected before separation by chromatography through techniques such as UV-Vis spectroscopy or fluorescence spectroscopy. These techniques can provide information about the absorption or emission properties of the pigments present in a sample.
o0o
The temperature of the room could be an uncontrolled variable in paper chromatography of pigments, as it can affect the rate at which the solvents evaporate and the separation of the pigments on the paper. Temperature fluctuations could lead to inconsistent results in the chromatography process.
Distillation
Hundreds of different pigments can be identified through the separation process using techniques like chromatography or spectrophotometry. Pigments such as chlorophyll a, chlorophyll b, carotenoids, and anthocyanins are commonly detected in plants.
Yes, the presence of different pigments can be detected before separation by chromatography through techniques such as UV-Vis spectroscopy or fluorescence spectroscopy. These techniques can provide information about the absorption or emission properties of the pigments present in a sample.
Chromatography will separate the pigments in the plant extract based on their different affinities for the stationary and mobile phases. This separation will allow for the identification and quantification of individual pigments within the extract.
Factors that affect leaf chromatography include the polarity of the solvent used, the size and shape of the molecules being separated, the pH of the solvent, and the temperature at which the chromatography is performed. These factors can impact the rate at which the molecules move through the chromatography medium and the resolution of the separation.
Yes they do affect the absorption of light in plants.
The separation of pigments occurs due to differences in their solubility and interactions with the medium they are in, such as a solvent or gel. Each pigment has distinct chemical properties, leading to varying rates of migration when subjected to processes like chromatography. As the solvent moves through the medium, more soluble pigments travel further, while less soluble ones remain closer to the origin, resulting in distinct bands or spots of color. This phenomenon allows for the identification and analysis of the individual pigments present in a mixture.
Separation does not affect ownership. Only the parties or the court can change the ownership.
The government would have become a tyranny if there was no separation of powers
Factors that affect the rate of photosynthesis include light intensity, carbon dioxide concentration, temperature, water availability, and the presence of chlorophyll and other pigments. Changes in any of these factors can influence the rate at which plants can convert light energy into chemical energy.