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In each complete run of the Calvin Cycle, six molecules of carbon dioxide eventually produce one molecule of glucose (C6H12O6). In each "turn" of the cycle, the 5-carbon molecule ribulose biphosphate (RuBP) bonds with an incoming carbon dioxide molecule to create a 6-carbon molecule. This 6-carbon molecule breaks into two 3-carbon molecules of phosphoglycerate (PGA). The energy stored in ATP and NADPH is used to attach phosphate groups to the PGA. Eventually, there are 12 molecules of glyceraldehyde phosphate (also known as phosphoglyceraldehyde or PGAL). Two of these molecules are removed from the cycle to make one glucose molecule. The remaining PGAL molecules use ATP energy to reform six RuBP molecules, and thus start the cycle again. Remember that a different reaction-specific enzyme catalyzes each step in this process.

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