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Gene splicing means cutting of gene in fragments and rejoining them according to need There are two phenomenon by which gene splicing occur one is natural i.e. post transcriptional modification and other is artifically or by chemical agent used generally in recombinant technology
POST TRANSCRIPTIONAL MODIFICATION: Gene splicing is a post-transcriptional modification in which a single gene can code for multiple proteins. Gene Splicing is done in eukaryotes, prior to mRNA translation, by the differential inclusion or exclusion of regions of pre-mRNA. Gene splicing is an important source of protein diversity. During a typical gene splicing event, the pre-mRNA transcribed from one gene can lead to different mature mRNA molecules that generate multiple functional proteins. Thus, gene splicing enables a single gene to increase its coding capacity, allowing the synthesis of protein isoforms that are structurally and functionally distinct. Gene splicing is observed in high proportion of genes. In human cells, about 40-60% of the genes are known to exhibit alternative splicing.
Gene Splicing Mechanism
There are several types of common gene splicing events. These are the events that can simultaneously occur in the genes after the mRNA is formed from the transcription step of the central dogma of molecular Biology.
Exon Skipping: This is the most common known gene splicing mechanism in which exon(s) are included or excluded from the final gene transcript leading to extended or shortened mRNA variants. The exons are the coding regions of a gene and are responsible for producing proteins that are utilized in various cell types for a number of functions.
Intron Retention: An event in which an intron is retained in the final transcript. In humans 2-5 % of the genes have been reported to retain introns. The gene splicing mechanism retains the non-coding (junk) portions of the gene and leads to a demornity in the protein structure and functionality.
Alternative 3' splice site and 5' splice site: Alternative gene splicing includes joining of different 5' and 3' splice site. In this kind of gene splicing, two or more alternative 5' splice site compete for joining to two or more alternate 3' splice site.
A gene-sized fragment of DNA is isolated from another organism. The bases of the fragment are spliced (joined) to the bases of the molecule by using a chemical called ligase.
What else can I help you with?
How does the cell get the instructions for building a protein from the nucleus out to the cytoplasm?
m-RNA copy the information through transcription.Then m-RNA comes to cytoplasm.
In eukaryotic cells the mRNA is processed and migrates from the to the .?
The mRNA produced by transcription is similar to a rough cut of a film that needs a bit of editing. A specialized nucleotide is added to the beggining of each mRNA molecule, which forms a cap. It helps the mRNA starand bind to a ribosome and prevents the strand from being broken down to fast. The end of the mRNA molecule gets a string of A nucleotides, called the tail, that helps the mRNA molecule exit the nucleus.
Which activity happens during M phase?
Cell division gradpoint
What does 'm represent in mRNA?
m stands for messenger, mRNA is messenger RNA
Which amino acid does GTT carry?
glutamine because GTT is the t-RNA and CAA is the m-RNA
Which happens first messager RNA or transfer RNA?
Messenger RNA (mRNA) is synthesized during transcription in the nucleus before transfer RNA (tRNA) comes into play during translation in the cytoplasm. So, mRNA is produced first in the sequence of events.
How does the cell get the instructions for building a protein from the nucleus out to the cytoplasm?
m-RNA copy the information through transcription.Then m-RNA comes to cytoplasm.
Rna that makes up the ribosomes clamps onto mrna and uses its information to assemble amino acids in the correct order?
M-RNA copies the message of the DNA of the gene. m-RNA leaves the nucleus and travels to the ribosome (r-RNA) where the t-RNA brings amino acids which are assembles in the order brought by the m-RNA.
What are the 3 main kinds of rna?
The three main kinds of RNA are messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). mRNA carries genetic information from the DNA to the ribosome for protein synthesis, tRNA brings amino acids to the ribosome during translation, and rRNA forms the core structure of the ribosome and catalyzes protein synthesis.
Which activity happens during the ''m' phase?
cell division
In eukaryotic cells the mRNA is processed and migrates from the to the .?
The mRNA produced by transcription is similar to a rough cut of a film that needs a bit of editing. A specialized nucleotide is added to the beggining of each mRNA molecule, which forms a cap. It helps the mRNA starand bind to a ribosome and prevents the strand from being broken down to fast. The end of the mRNA molecule gets a string of A nucleotides, called the tail, that helps the mRNA molecule exit the nucleus.
Which activity happens during M phase?
Cell division gradpoint
Where is m RNA in the eukaryote cells?
ribosome
What does 'm represent in mRNA?
m stands for messenger, mRNA is messenger RNA
Where do you find a anticodon?
Codon is found on the messenger RNA(m RNA).During translation, the codons on the m RNA are read by the ribosome and amino acid corresponding to the codon is added. when ribosome encounters a stop codon (UAG,UGA and UAA) translation terminates.
What are two types of RNA?
There are actually three types of RNA.The transfer RNA (tRNA), which delivers the anticodon and the amino acid,The messenger RNA (mRNA), which contains the codonThe ribosomal RNA (rRNA), which combines with proteins to form the ribosome.
How does m-RNA read DNA-CCAGTT?
The messenger RNA (or mRNA) compliment to a DNA strand of CCAGTT would be GGUCAA.
