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Using two different sizes of cuvettes for the same solution can lead to variations in absorbance readings due to differences in path length. According to Beer-Lambert law, absorbance is directly proportional to the path length, so a longer path length will result in higher absorbance for the same concentration. Consequently, comparing absorbance values obtained from different sized cuvettes without proper adjustments can yield misleading results. It is essential to standardize the cuvette size when conducting comparative analyses.

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1w ago

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