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The largest vacuole is typically found in plant cells, specifically in mature cells of certain plants, such as those in the family of flowering plants (angiosperms). These vacuoles can occupy up to 90% of the cell's volume, serving functions such as storage of nutrients, waste products, and maintaining turgor pressure to support the plant structure. In some cases, the giant cell of the green alga Valonia has been noted for its particularly large vacuole, showcasing extreme examples of vacuolar size in nature.

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