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Since CsCl is a kind of heavy salt, it forms density gradients when centrifugation. When sedimentation equilibrium, DNA moves to the position where meets its density. The density of DNA is related to its GC%, by analytical ultracentrifugation, we can read the GC levels of the DNA from its position in the solution.
Please refer to the article for detail:
Using analytical ultracentrifugation to study compositional variation in vertebrate genomes (Euro Biophys J. 2003 32: pp418-426)
What else can I help you with?
How many markers are used to make a dna profile?
In forensic DNA analysis, a DNA profile is typically created using 20 genetic markers. These markers are specific regions of the genome that vary between individuals and are used to establish a unique genetic fingerprint for identification purposes.
How many cells are needed to profile DNA?
10
What DNA fingerprinting is and what technique is used?
DNA fingerprinting is a method used to identify an individual based on their unique genetic makeup. The technique used involves extracting DNA from a sample, such as blood or hair, and then analyzing specific regions of the DNA called microsatellites or short tandem repeats to create a unique genetic profile. This profile can then be compared to other samples to determine genetic relatedness or identity.
Which process involving DNA creates a DNA pattern or fingerprint unique to an individual person?
The process involving DNA that creates a unique DNA pattern or fingerprint for an individual person is called DNA profiling or DNA fingerprinting. This technique analyzes specific regions of an individual's DNA to create a unique genetic profile that can be used for identification purposes.
DNA samples for human DNA fingerprinting can be obtained from?
DNA samples for human DNA fingerprinting can be obtained from a variety of sources, including blood, saliva, hair follicles, and skin cells. These samples contain DNA that can be used for analysis and comparison to create a unique genetic profile for each individual.
Why is hair used as evidence?
Hair can be used for a DNA profile.
How many markers are used to make a dna profile?
In forensic DNA analysis, a DNA profile is typically created using 20 genetic markers. These markers are specific regions of the genome that vary between individuals and are used to establish a unique genetic fingerprint for identification purposes.
Can a mouth swab sent away for DNA testing determine the age of the person it was collected from?
No, it can only be used to generate a DNA profile.
What is the role of Cscl and ethidium bromide in plasmid purification?
The CsCl forms a gradient and the molecules migrate according to their density until they float at their individual isopycnic points (the point in the gradient that equals the buoyant density of the molecule). However, plasmid DNA and contaminating chromosome have about the same density and cannot be separated easily. This is rectified, however, by the addition of ethidium bromide. Density is a function of AT/GC ratio, but it is also a function of conformation. For supercoiled DNA, there is more DNA per unit volume than for relaxed DNA. Intercalation of ethidium bromide into DNA causes the helix to unwind (negative supercoiling) and become more relaxed. However, negative supercoiling only relaxes the DNA to a point. Further unwinding induces supercoiling in the opposite direction. When the DNA is circular and the ends are connected, the plasmid "kinks up" into a very tight knot. Thus, ethidium bromide causes the plasmid density to be increased.
Are genetic fingerprints and DNA fingerprints the same?
Yes, genetic fingerprints and DNA fingerprints refer to the same thing - the unique genetic profile of an individual that can be used for identification purposes. This profile is based on variations in an individual's DNA sequence.
A pattern produced from fragments of the DNA of a specific person?
A DNA profile is a pattern produced from fragments of an individual's DNA that can be used for identification purposes. This profile is unique to each person, except identical twins, making it a powerful tool for forensic investigations and paternity testing. DNA profiles are created by analyzing specific genetic markers or regions of the DNA that vary between individuals.
How many cells are needed to profile DNA?
10
What DNA fingerprinting is and what technique is used?
DNA fingerprinting is a method used to identify an individual based on their unique genetic makeup. The technique used involves extracting DNA from a sample, such as blood or hair, and then analyzing specific regions of the DNA called microsatellites or short tandem repeats to create a unique genetic profile. This profile can then be compared to other samples to determine genetic relatedness or identity.
What is DNA profile?
DNA profiling is a technique employed by forensic scientists to assist in the identification of individuals on the basis of their respective DNA profiles.
Did the US develop a DNA Profile for Osama bin Laden?
No
What is csf1po?
CSF1PO is one of the 13 core loci used by the United States government when performing a DNA profile. As for what it stands for I don't know.
Which process involving DNA creates a DNA pattern or fingerprint unique to an individual person?
The process involving DNA that creates a unique DNA pattern or fingerprint for an individual person is called DNA profiling or DNA fingerprinting. This technique analyzes specific regions of an individual's DNA to create a unique genetic profile that can be used for identification purposes.
