In simple terms, it's a process where they essentially "unzip" the genes of living things into their billions of chemical building blocks to determine the exact order of the chemicals. It's useful for finding inherited diseases, identifying family lineage, etc since certain illnesses and traits have been found to be linked to certain chemical strands in DNA. It also helps when people are trying to breed certain traits into an animal or plant species. They can use DNA sequencing to find specimens that have the traits they want as a dominant trait in their DNA and cultivate them in such a way as to encourage that trait to emerge most of the time.
Single molecule real time sequencing was developed by Pacific Biosciences and uses synthesis technology. It is a parrallelized single molecule DNA sequencing.
A common approach to DNA sequencing is through a process called Sanger sequencing, named after its inventory, Frederick Sanger. To describe the process simply, a sample of purified DNA is treated with a solution of enzymes, nucleotides, and terminators to duplicate the strands of DNA. As the DNA is being copied, it uses the nucleotides to form new strands of DNA and sometimes will add a terminator which stops the duplication process at varying lengths. The terminators are labeled with a radioactive or fluorescent chemical which allows them to be detected by a scanning machine. In capillary electrophoresis, the mixture of varying length DNA is separated in a very narrow tube and as each terminator passes by the detector, the sequence of the DNA bases can be read. For a more detailed description of the mechanics of Sanger sequencing, an internet search will yield many results.
The types of DNA sequencing are whole-genome sequencing which maps entire DNA sequences, targeted sequencing which focuses on specific genomic regions, and RNA sequencing which identifies gene expression levels.
Some methods that are sequencing DNA is utilizing labeled nucleotides for corporation into a copy of a piece of DNA. The DNA segment to be copied, called the template DNA, is separated into two strands by heating.
DNA fragments produced by automated DNA sequencing are identified using fluorescent dyes or radioisotopes attached to the nucleotides in the DNA sequence. The sequencing machine reads the colors emitted by the dyes or the radioactive signals to determine the order of bases in the DNA fragment.
Single molecule real time sequencing was developed by Pacific Biosciences and uses synthesis technology. It is a parrallelized single molecule DNA sequencing.
DNA sequencing was first discovered by Fredrick sanger in 1950s
Sequencing DNA rapidly
A common approach to DNA sequencing is through a process called Sanger sequencing, named after its inventory, Frederick Sanger. To describe the process simply, a sample of purified DNA is treated with a solution of enzymes, nucleotides, and terminators to duplicate the strands of DNA. As the DNA is being copied, it uses the nucleotides to form new strands of DNA and sometimes will add a terminator which stops the duplication process at varying lengths. The terminators are labeled with a radioactive or fluorescent chemical which allows them to be detected by a scanning machine. In capillary electrophoresis, the mixture of varying length DNA is separated in a very narrow tube and as each terminator passes by the detector, the sequence of the DNA bases can be read. For a more detailed description of the mechanics of Sanger sequencing, an internet search will yield many results.
People not versed in DNA sequencing.
The types of DNA sequencing are whole-genome sequencing which maps entire DNA sequences, targeted sequencing which focuses on specific genomic regions, and RNA sequencing which identifies gene expression levels.
Dideoxynucleotides are used in Sanger DNA sequencing to stop the DNA replication process at specific points, allowing for the determination of the sequence of nucleotides in a DNA strand.
When looking for information about the sequence of DNA then there is information relating to the concept of genetic sequencing available from Wikipedia. The site offers about DNA sequencing with links that relate to other facts and information on the different aspects of genetic sequencing.
by DNA fingerprinting method , DNA-DNA hybirdization or DNA sequencing. to know the sequence of DNA
It is common knowledge that pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle.
In DNA sequencing, Adenine and Guanine are known as "base pairs", and are purines, which form the building blocks of DNA and RNA. Guanine combines with Adenine in DNA sequencing.
Some methods that are sequencing DNA is utilizing labeled nucleotides for corporation into a copy of a piece of DNA. The DNA segment to be copied, called the template DNA, is separated into two strands by heating.