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What is a backmutation?

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Anonymous

 ∙ 10y ago
Updated: 10/27/2022

A backmutation is a mutation in genetics which restores the original sequence and the original phenotype.

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 ∙ 10y ago

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When do you take the ames test?

The ames test is a basic toxicological test that can be used to determine if a substance is potentially genotoxic. It is a very easy and cheap test that can be set up in most labs. The test looks for gene mutations in bacteria, normally Salmonella typhimurium. A strain of S. typhimurium with a mutation that causes it to be unable to synthezise histidine (amino acid) is plated along with the substance to be tested. The mutation in the bacteria can easily be backmutated, thus, any substance with a genotoxic (mutagenic) ability can cause the bacteria to backmutate to a state where it can again syntesize histidine. S. typh on agar plate without histidine will not be able to grow, and thus, no colony forming units (cfu) will be seen. S. typh on agar plate, add potentially genotoxic substance onto agar (normally on paper disc), the substance will filter through agar, and if mutagenic, can cause backmutation in the bacteria which will then be able to synthesize histidine, resulting in growt of cfu's. The results seen in the ames test can give an idea of the substances potential for genotoxicity. If the substance has the ability to cause a mutation in the bacteria, it might be able to cause mutations in humans as well, and should therefore undergo further testing to determine whether it is safe or not.

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