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A cell line usually refers to an immortal culture of cells derived from a specific tussue. Immortal means that they do not die after a certain number of divisions, like 'normal' cells would. Still, there are finite- and continous cell lines. These cells can be of human origin (e.g. MCF7 cells), simian (COS), rodent (CHO cells), and many more. They are derived from many different tissues, like epithelial and neuronal, and can find their origins in organs such as kidneys, ovaria, etc. Researchers keep cell lines in a media that contains nutrients like sugars, proteins and anti biotics and they are usually kept at a temperature of 37o C in an atmosphere of humidified air with 5% CO2. Typical cell lines such as aforementioned cover a petri dish (92 mm across) from a density of 5% to 100% in three to four days under optimal conditions. Cell lines often replace laboratory animals and can be used for a number of different kinds of research.
Examples: yeast cells are in use to produce human recombinant insulin for example, so that diabetics don't have to rely on bovine insuline as that was the only available insuline for years. E.coli DH5a cells are frequently used to amplify amounts of DNA because of their rapid rate of cell division and low requirements to the environment they are kept and grown in. Mammalian cell lines can be used for anything from hosting and producing amounts of viral vectors for research, to characterisation of induction of gene expression by free radicals for instance. Cell lines are also frequently transfected with foreign DNA in order to investigate function of genes and their proteins.
What else can I help you with?
What is NIH T3T cell line?
I belive you mean the NIH 3T3 cell line which is the mouse embryonic fibroblast cell line, capable of differentiating into multiple cell types - adipocytes, myocytes or chondrocytes.
How is positive terminal represent in the symbol of cell?
in digs.the longer line(in height) represents the positive terminal.
During what the sister chromatids line up in the midplane of the cell?
In Metaphase the chromatids line up at the equator/midplane of the cell.
Do the chromosomes line up in the center of the cell?
They line up during metaphase of mitosis and meiosis
What stage of meiosis is when chromosomes line up in the center of the cell?
Chromosomes line up in the middle of the cell during meiosis.
What is the part of the cell that stores power?
line line cell
What is NIH T3T cell line?
I belive you mean the NIH 3T3 cell line which is the mouse embryonic fibroblast cell line, capable of differentiating into multiple cell types - adipocytes, myocytes or chondrocytes.
What is animal cell line?
A cell wall protects the Animal Cell.
What stage of mitosis is when the chromosomes line up in the middle of the cell?
the cell is in the stage of metaphase.
How is positive terminal represent in the symbol of cell?
in digs.the longer line(in height) represents the positive terminal.
In mitosis chromatids line up in the middle of the cell?
Chromatids line up during the metaphase of the cell cycle.
In mitosis chromatide line up in the middle of the cell during?
Chromatids line up during the metaphase of the cell cycle.
How do you enter two line in same cell in Excel?
Press Alt+Enter to add another line within a sincle cell. Type your first line, then press Alt+Enter to move to the second line. In the formula window you will see only the first line unless you use the up and down arrow keys. In the cell you will see both lines. If your lines do not wrap and show up in the cell, format the cell with Cell Wrap turned on.
What is a stem line?
a stem cell line is a group of stem cells that has the ability to produce one specific type of cell. for example, one stem cell line could have the ability to produce a kidney, while another line could produce a liver.
Do you need to add a line to get a cell phone?
You do not need any land line at all in order to own and use a cell phone.
Where are chromosomes located in relation to the cell?
The chromosomes line up on what is called the metaphase plate. This is an imaginary line that splits the cell in two.
Example of the cell membrane?
The thin, outer line surrounding the cell. It helps bring items in and out of the cell
