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Plant tissue culture is a technique of producing clones of plant under controlled sterile condition. IN plant tissue culture, an explant is used to produce the whole plant in quick succession of time. Various steps include in the process are: * Prepare a suitable nutrient medium with carbon, nitrogen source along with the growth regulators. * Isolation of the explants (from the original plant) to be used. * Sterilization of the whole equipments like; vessels, media, explants, test tubes. * inoculation * incubation * hardening * Establishment in the field.

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